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Development of imaging scaffolds for cryo-electron microscopy

Yeates, Todd O. and Agdanowski, Matthew P. and Liu, Yuxi (2020) Development of imaging scaffolds for cryo-electron microscopy. Current Opinion in Structural Biology, 60 . pp. 142-149. ISSN 0959-440X. doi:10.1016/

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Following recent hardware and software developments, single particle cryo-electron microscopy (cryo-EM) has become one of the most popular structural biology tools. Many targets, such as viruses, large protein complexes and oligomeric membrane proteins, have been resolved to atomic resolution using single-particle cryo-EM, which relies on the accurate assignment of particle location and orientation from intrinsically noisy projection images. The same image processing procedures are more challenging for smaller proteins due to their lower signal-to-noise ratios. Consequently, though most cellular proteins are less than 50 kDa, so far it has been possible to solve cryo-EM structures near that size range for only a few favorable cases. Here we highlight some of the challenges and recent efforts to break through this lower size limit by engineering large scaffolds to rigidly display multiple small proteins for imaging. Future design efforts are noted.

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Liu, Yuxi0000-0003-1439-9000
Additional Information:© 2020 Elsevier Ltd. Available online 14 February 2020. This work was supported by N.I.H. grant R01 GM129854 (to TOY). Author contributions: TOY, YL, and MA contributed equally to writing and revising the manuscript. Conflict of interest statement: Nothing declared.
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NIHR01 GM129854
Record Number:CaltechAUTHORS:20200430-130923754
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Official Citation:Todd O Yeates, Matthew P Agdanowski, Yuxi Liu, Development of imaging scaffolds for cryo-electron microscopy, Current Opinion in Structural Biology, Volume 60, 2020, Pages 142-149, ISSN 0959-440X, (
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:102933
Deposited By: Tony Diaz
Deposited On:30 Apr 2020 20:28
Last Modified:16 Nov 2021 18:16

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