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Modelling T-cell memory by genetic marking of memory T cells in vivo

Jacob, Joshy and Baltimore, David (1999) Modelling T-cell memory by genetic marking of memory T cells in vivo. Nature, 399 (6736). pp. 593-597. ISSN 0028-0836. doi:10.1038/21208.

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Immunological memory is the ability of the immune system to respond with enhanced vigour to pathogens that have been encountered in the past. Following infection or immunization, most effector T cells undergo apoptotic cell death, but a small fraction of these cells, proportional to the early antigen load and initial clonal burst size, persist in the host as a stable pool of memory T cells. The existence of immunological memory has been recognized for over 2,000 years, but our understanding of this phenomenon is limited, primarily because memory lymphocytes cannot be unequivocally identified as they lack specific, permanent markers. Here we have developed a transgenic mouse model system whereby memory T cells and their precursors can be irreversibly marked with a reporter gene and thus can be unambiguously identified. Adoptive transfer of marked CD8⁺ T cells specific for lymphocytic choriomeningitis virus protected naive recipients following viral challenge, demonstrating that we have marked memory T cells. We also show that cytotoxic effector lymphocytes that develop into memory T cells can be identified in the primary response.

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Baltimore, David0000-0001-8723-8190
Additional Information:© 1999 Macmillan Magazines Ltd. Received 25 January 1999; Accepted 09 April 1999; Issue Date 10 June 1999. We thank C. Roman, I. Stancovski, S. Cherry, K. Alexandroplous, M. Scott, G.Kelsoe, R. Welsh, L. Selin, R. Ahmed and members of the Baltimore laboratory for useful discussions. We also thank T. Ley for p687 plasmid; R. Welsh, R. Ahmed and J. Carlos de la Torre for LCM viral stocks; D. Kioussis for the VahCD2 transgenic construct; M. Brasch for pBS185 and pBS302 plasmids; and A.Nagy for the hCMV-Cre transgenic mice. J.J. Thanks H. Rayburn and W. Sha for teaching him pronuclear injection and transgenesis, and M. Scott for help with adoptive transfers. J.J. was a recipient of a postdoctoral fellowship from the Helen Hay Whitney foundation and is currently a special fellow of the Leukemia Society of America.
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Helen Hay Whitney FoundationUNSPECIFIED
Leukemia Society of AmericaUNSPECIFIED
Issue or Number:6736
Record Number:CaltechAUTHORS:20200506-153313792
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Official Citation:Jacob, J., Baltimore, D. Modelling T-cell memory by genetic marking of memory T cells in vivo. Nature 399, 593–597 (1999).
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:103051
Deposited By: Tony Diaz
Deposited On:06 May 2020 22:38
Last Modified:16 Nov 2021 18:17

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