Xu, Yang and Davidson, Laurie and Alt, Frederick W. and Baltimore, David (1996) Deletion of the Igκ Light Chain Intronic Enhancer/Matrix Attachment Region Impairs but Does Not Abolish VκJκ Rearrangement. Immunity, 4 (4). pp. 377-385. ISSN 1074-7613. doi:10.1016/s1074-7613(00)80251-4. https://resolver.caltech.edu/CaltechAUTHORS:20200507-112119157
Full text is not posted in this repository. Consult Related URLs below.
Use this Persistent URL to link to this item: https://resolver.caltech.edu/CaltechAUTHORS:20200507-112119157
Abstract
Roles of the κ intronic enhancer (iEκ) and its asssociated matrix attachment region (MAR) during B cell development were examined using mutant embryonic stem (ES) cell lines in which the entire region on both chromosomes was replaced with either a recombined LoxP site (EκND) or the PGK–neomycin resistance (PGK-neor) gene (EκNI). B cells derived from EκND ES cells had greatly impaired VκJκ rearrangement, normal levels of κ expression, and κ:λ ratios of 1:1 instead of the usual 10:1. Furthermore, λ-producing hybridomas derived from EκND cells displayed little κ rearrangement. Thus, the MAR and iEκ are quantitatively significant for κ rearrangement but not necessary. In addition, little VκJκ rearrangement could be detected in B cells derived from EκNI ES cells, demonstrating that an inserted PGK–neor gene dominantly suppresses VκJκ rearrangement.
| Item Type: | Article | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Related URLs: |
| ||||||||||
| ORCID: |
| ||||||||||
| Additional Information: | © 1996 Cell Press. Received 18 January 1996, Revised 13 March 1996. Correspondence should be addressed to D. B. We thank Dr. E. Selsing for the RS probe; S. Cherry and Dr. C. Roman for critically reading the manuscript. This work was supported by a National Institutes of Health grant to D. B., National Institutes of Health grant AI-20047 to F. W. A., and the Howard Hughes Medical Institute. Y. X. was supported by a fellowship from the Cancer Research Fund of the Damon Runyon–Walter Winchell Foundation. D. B. is an American Cancer Society Research Professor. | ||||||||||
| Funders: |
| ||||||||||
| Issue or Number: | 4 | ||||||||||
| DOI: | 10.1016/s1074-7613(00)80251-4 | ||||||||||
| Record Number: | CaltechAUTHORS:20200507-112119157 | ||||||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20200507-112119157 | ||||||||||
| Official Citation: | Yang Xu, Laurie Davidson, Frederick W Alt, David Baltimore, Deletion of the Igκ Light Chain Intronic Enhancer/Matrix Attachment Region Impairs but Does Not Abolish VκJκ Rearrangement, Immunity, Volume 4, Issue 4, 1996, Pages 377-385, ISSN 1074-7613, https://doi.org/10.1016/S1074-7613(00)80251-4. (http://www.sciencedirect.com/science/article/pii/S1074761300802514) | ||||||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | ||||||||||
| ID Code: | 103072 | ||||||||||
| Collection: | CaltechAUTHORS | ||||||||||
| Deposited By: | Tony Diaz | ||||||||||
| Deposited On: | 08 May 2020 17:54 | ||||||||||
| Last Modified: | 16 Nov 2021 18:18 |
Repository Staff Only: item control page
