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Deletion of the Igκ Light Chain Intronic Enhancer/Matrix Attachment Region Impairs but Does Not Abolish VκJκ Rearrangement

Xu, Yang and Davidson, Laurie and Alt, Frederick W. and Baltimore, David (1996) Deletion of the Igκ Light Chain Intronic Enhancer/Matrix Attachment Region Impairs but Does Not Abolish VκJκ Rearrangement. Immunity, 4 (4). pp. 377-385. ISSN 1074-7613. https://resolver.caltech.edu/CaltechAUTHORS:20200507-112119157

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Abstract

Roles of the κ intronic enhancer (iEκ) and its asssociated matrix attachment region (MAR) during B cell development were examined using mutant embryonic stem (ES) cell lines in which the entire region on both chromosomes was replaced with either a recombined LoxP site (EκND) or the PGK–neomycin resistance (PGK-neor) gene (EκNI). B cells derived from EκND ES cells had greatly impaired VκJκ rearrangement, normal levels of κ expression, and κ:λ ratios of 1:1 instead of the usual 10:1. Furthermore, λ-producing hybridomas derived from EκND cells displayed little κ rearrangement. Thus, the MAR and iEκ are quantitatively significant for κ rearrangement but not necessary. In addition, little VκJκ rearrangement could be detected in B cells derived from EκNI ES cells, demonstrating that an inserted PGK–neor gene dominantly suppresses VκJκ rearrangement.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1016/s1074-7613(00)80251-4DOIArticle
ORCID:
AuthorORCID
Baltimore, David0000-0001-8723-8190
Additional Information:© 1996 Cell Press. Received 18 January 1996, Revised 13 March 1996. Correspondence should be addressed to D. B. We thank Dr. E. Selsing for the RS probe; S. Cherry and Dr. C. Roman for critically reading the manuscript. This work was supported by a National Institutes of Health grant to D. B., National Institutes of Health grant AI-20047 to F. W. A., and the Howard Hughes Medical Institute. Y. X. was supported by a fellowship from the Cancer Research Fund of the Damon Runyon–Walter Winchell Foundation. D. B. is an American Cancer Society Research Professor.
Funders:
Funding AgencyGrant Number
NIHAI-20047
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
Damon Runyon Cancer Research FoundationUNSPECIFIED
American Cancer SocietyUNSPECIFIED
Issue or Number:4
Record Number:CaltechAUTHORS:20200507-112119157
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20200507-112119157
Official Citation:Yang Xu, Laurie Davidson, Frederick W Alt, David Baltimore, Deletion of the Igκ Light Chain Intronic Enhancer/Matrix Attachment Region Impairs but Does Not Abolish VκJκ Rearrangement, Immunity, Volume 4, Issue 4, 1996, Pages 377-385, ISSN 1074-7613, https://doi.org/10.1016/S1074-7613(00)80251-4. (http://www.sciencedirect.com/science/article/pii/S1074761300802514)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:103072
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:08 May 2020 17:54
Last Modified:08 May 2020 17:54

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