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Localization, interaction, and RNA binding properties of the V(D)J recombination-activating proteins RAG1 and RAG2

Spanopoulou, Eugenia and Cortes, Patricia and Shih, Charles and Huang, Chun-Ming and Silver, Daniel P. and Svec, Pamela and Baltimore, David (1995) Localization, interaction, and RNA binding properties of the V(D)J recombination-activating proteins RAG1 and RAG2. Immunity, 3 (6). pp. 715-726. ISSN 1074-7613. doi:10.1016/1074-7613(95)90061-6.

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The RAG1 and RAG2 gene products are indispensable for activating somatic rearrangement of antigen receptor gene segments. The two proteins form a stable complex in primary thymocytes as well as when expressed in adherent cells. In both cell types, most cells localize RAG proteins at the periphery of the nucleus. However, when overexpressed in fibroblast cells, RAG1 is found largely in the nucleolus. Nucleolar localization of RAG1 is mediated by several domains containing stretches of basic amino acids, indicating that RAG1 has affinity for RNA or ssDNA. The RAG1 interacting proteins SRP1 and Rch1 directly bind to the nuclear localization signals of RAG1, which mediate the nuclear and nucleolar translocation of the protein. RAG1 appears to have a binary structure, each half containing multiple regions that can act as NLSs, binding sites for the SRP1/Rch1 family, and RNA binding domains.

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Baltimore, David0000-0001-8723-8190
Additional Information:© 1995 by Cell Press. Received 11 September 1995, Revised 11 October 1995. Both E. S. and P. C. made key independent contributions. We thank Drs. T. Meier for the anti-Nopp140 serum; M. Scott for guiding E. S. in immunofluorescence techniques; B. Mayer for donating anti-GST Localization and Properties of RAG1 and RAG2 Proteins 725 sera and pERG vector; Y. W. Choi for hospitality; and M. Konarska, R. Andino, A. Hodtsev, and D. Alexandropoulos for several valuable suggestions. E. S. was initially supported by a European Molecular Biology Organization long-term fellowship and later by a Cancer Research Institute fellowship. P. C. was supported by a fellowship from the Irvington Institute and is currently supported by the Lymphoma Research Foundation of America. This work was supported by National Institutes of Health grant CA54162 to D. B.
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European Molecular Biology Organization (EMBO)UNSPECIFIED
Cancer Research InstituteUNSPECIFIED
Lymphoma Research Foundation of AmericaUNSPECIFIED
Issue or Number:6
Record Number:CaltechAUTHORS:20200507-153522640
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Official Citation:Eugenia Spanopoulou, Patricia Cortes, Charles Shih, Chun-Ming Huang, Daniel P. Silver, Pamela Svec, David Baltimore, Localization, interaction, and RNA binding properties of the V(D)J recombination-activating proteins RAG1 and RAG2, Immunity, Volume 3, Issue 6, 1995, Pages 715-726, ISSN 1074-7613, (
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:103081
Deposited By: Tony Diaz
Deposited On:08 May 2020 15:43
Last Modified:16 Nov 2021 18:18

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