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Funneled angle landscapes for helical proteins

Kozak, John J. and Gray, Harry B. and Garza-López, Roberto A. (2020) Funneled angle landscapes for helical proteins. Journal of Inorganic Biochemistry, 208 . Art. No. 111091. ISSN 0162-0134. PMCID PMC7453651. doi:10.1016/j.jinorgbio.2020.111091.

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We use crystallographic data for four helical iron proteins (cytochrome c-b₅₆₂, cytochrome c′, sperm whale myoglobin, human cytoglobin) to calculate radial and angular signatures as each unfolds from the native state stepwise though four unfolded states. From these data we construct an angle phase diagram to display the evolution of each protein from its native state; and, in turn, the phase diagram is used to construct a funneled angle landscape for comparison with the topography of its folding energy landscape. We quantify the departure of individual helical and turning regions from the areal, angular profile of corresponding regions of the native state. This procedure allows us to identify the similarities and differences among individual helical and turning regions in the early stages of unfolding of the four helical heme proteins.

Item Type:Article
Related URLs:
URLURL TypeDescription CentralArticle
Gray, Harry B.0000-0002-7937-7876
Garza-López, Roberto A.0000-0002-9363-1842
Additional Information:© 2020 Published by Elsevier Inc. Received 4 January 2020, Revised 14 April 2020, Accepted 16 April 2020, Available online 11 May 2020. We thank Devarajan (Dave) Thirumalai for very helpful comments. Work at Caltech was supported by the NIH (DK019038) and the Arnold and Mabel Beckman Foundation. Support at Pomona College was provided by the Howard Hughes Medical Institute Research Program and a Sontag Research Fellowship Award. The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
Funding AgencyGrant Number
Arnold and Mabel Beckman FoundationUNSPECIFIED
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
Sontag Research Fellowship AwardUNSPECIFIED
Subject Keywords:Protein folding; Cytochromes; Myoglobin
PubMed Central ID:PMC7453651
Record Number:CaltechAUTHORS:20200511-125453032
Persistent URL:
Official Citation:John J. Kozak, Harry B. Gray, Roberto A. Garza-López, Funneled angle landscapes for helical proteins, Journal of Inorganic Biochemistry, Volume 208, 2020, 111091, ISSN 0162-0134,
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:103106
Deposited By: Tony Diaz
Deposited On:11 May 2020 20:12
Last Modified:15 Feb 2022 18:38

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