CaltechAUTHORS
  A Caltech Library Service

Funneled angle landscapes for helical proteins

Kozak, John J. and Gray, Harry B. and Garza-López, Roberto A. (2020) Funneled angle landscapes for helical proteins. Journal of Inorganic Biochemistry, 208 . Art. No. 111091. ISSN 0162-0134. https://resolver.caltech.edu/CaltechAUTHORS:20200511-125453032

[img] PDF - Accepted Version
See Usage Policy.

1425Kb
[img] MS Word (Supplementary data. Angle Phase Diagrams for cyt c-b562 and h-Cygb. Funneled Angle Landscapes for cyt c' and sw-Mb) - Supplemental Material
See Usage Policy.

1020Kb

Use this Persistent URL to link to this item: https://resolver.caltech.edu/CaltechAUTHORS:20200511-125453032

Abstract

We use crystallographic data for four helical iron proteins (cytochrome c-b₅₆₂, cytochrome c′, sperm whale myoglobin, human cytoglobin) to calculate radial and angular signatures as each unfolds from the native state stepwise though four unfolded states. From these data we construct an angle phase diagram to display the evolution of each protein from its native state; and, in turn, the phase diagram is used to construct a funneled angle landscape for comparison with the topography of its folding energy landscape. We quantify the departure of individual helical and turning regions from the areal, angular profile of corresponding regions of the native state. This procedure allows us to identify the similarities and differences among individual helical and turning regions in the early stages of unfolding of the four helical heme proteins.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1016/j.jinorgbio.2020.111091DOIArticle
ORCID:
AuthorORCID
Gray, Harry B.0000-0002-7937-7876
Additional Information:© 2020 Published by Elsevier Inc. Received 4 January 2020, Revised 14 April 2020, Accepted 16 April 2020, Available online 11 May 2020. We thank Devarajan (Dave) Thirumalai for very helpful comments. Work at Caltech was supported by the NIH (DK019038) and the Arnold and Mabel Beckman Foundation. Support at Pomona College was provided by the Howard Hughes Medical Institute Research Program and a Sontag Research Fellowship Award. Declaration of competing interest: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
Funders:
Funding AgencyGrant Number
NIHDK019038
Arnold and Mabel Beckman FoundationUNSPECIFIED
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
Sontag Research Fellowship AwardUNSPECIFIED
Subject Keywords:Protein folding; Cytochromes; Myoglobin
Record Number:CaltechAUTHORS:20200511-125453032
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20200511-125453032
Official Citation:John J. Kozak, Harry B. Gray, Roberto A. Garza-López, Funneled angle landscapes for helical proteins, Journal of Inorganic Biochemistry, Volume 208, 2020, 111091, ISSN 0162-0134, https://doi.org/10.1016/j.jinorgbio.2020.111091. (http://www.sciencedirect.com/science/article/pii/S0162013420301197)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:103106
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:11 May 2020 20:12
Last Modified:02 Jun 2020 16:38

Repository Staff Only: item control page