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Poliovirus RNA synthesis utilizes an RNP complex formed around the 5′-end of viral RNA

Andino, Raul and Rieckhof, Gabrielle E. and Achacoso, Philip L. and Baltimore, David (1993) Poliovirus RNA synthesis utilizes an RNP complex formed around the 5′-end of viral RNA. EMBO Journal, 12 (9). pp. 3587-3598. ISSN 0261-4189. PMCID PMC413634. https://resolver.caltech.edu/CaltechAUTHORS:20200513-132321186

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Abstract

The structure of a ribonucleoprotein complex formed at the 5′‐end of poliovirus RNA was investigated. This complex involves the first 90 nucleotides of poliovirus genome which fold into a cloverleaf‐like structure and interact with both uncleaved 3CD, the viral protease‐polymerase precursor, and a 36 kDa ribosome‐associated cellular protein. The cellular protein is required for complex formation and interacts with unpaired bases in one stem‐loop of the cloverleaf RNA. Amino acids within the 3C protease which are important for RNA binding were identified by site‐directed mutagenesis and the crystal structure of a related protease was used to model the RNA binding domain within the viral 3CD protein. The physiologic importance of the ribonucleic‐protein complex is suggested by the finding that mutations that disrupt complex formation abolish RNA replication but do not affect RNA translation or stability. Based on these structural and functional findings we propose a model for the initiation of poliovirus RNA synthesis where an initiation complex consisting of 3CD, a cellular protein, and the 5′‐end of the positive strand RNA catalyzes in trans the initiation of synthesis of new positive stranded RNA.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1002/j.1460-2075.1993.tb06032.xDOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC413634PubMed CentralArticle
ORCID:
AuthorORCID
Baltimore, David0000-0001-8723-8190
Additional Information:© 1993 European Molecular Biology Organization. Received on May 19, 1993. Published: 1 September 1993. We are grateful to Drs Gerardo Kaplan for anti-3D antiserum, Douglas Black for technical advice, and Mark B. Feinberg, Alan Frankel, Laura Baxter and Shelley D. Suggett for their useful comments on the manuscript. This work was supported by Public Health Service grant no. AI22346 to D. Baltimore.
Funders:
Funding AgencyGrant Number
NIHAI22346
Subject Keywords:initiation complex; poliovirus; protease-polymerase; ribonucleoprotein; RNA synthesis
Issue or Number:9
PubMed Central ID:PMC413634
Record Number:CaltechAUTHORS:20200513-132321186
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20200513-132321186
Official Citation:Andino, R., Rieckhof, G., Achacoso, P. and Baltimore, D. (1993), Poliovirus RNA synthesis utilizes an RNP complex formed around the 5′‐end of viral RNA.. The EMBO Journal, 12: 3587-3598. doi:10.1002/j.1460-2075.1993.tb06032.x
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:103180
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:13 May 2020 22:28
Last Modified:13 May 2020 22:28

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