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Crystal structure of the phosphotyrosine recognition domain SH2 of v-src complexed with tyrosine-phosphorylated peptides

Waksman, Gabriel and Kominos, Dorothea and Robertson, Scott C. and Pant, Nalin and Baltimore, David and Birge, Raymond B. and Cowburn, David and Hanafusa, Hidesaburo and Mayer, Bruce J. and Overduin, Michael and Resh, Marilyn D. and Rios, Carlos B. and Silverman, Lauren and Kuriyan, John (1992) Crystal structure of the phosphotyrosine recognition domain SH2 of v-src complexed with tyrosine-phosphorylated peptides. Nature, 358 (6388). pp. 646-653. ISSN 0028-0836. https://resolver.caltech.edu/CaltechAUTHORS:20200513-153340587

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Abstract

Three-dimensional structures of complexes of the SH2 domain of the v-src oncogene product with two phosphotyrosyl peptides have been determined by X-ray crystallography at resolutions of 1.5 and 2.0 Å, respectively. A central antiparallel β-sheet in the structure is flanked by two α-helices, with peptide binding mediated by the sheet, intervening loops and one of the helices. The specific recognition of phosphotyrosine involves amino–aromatic interactions between lysine and arginine side chains and the ring system in addition to hydrogen-bonding interactions with the phosphate.


Item Type:Article
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URLURL TypeDescription
https://doi.org/10.1038/358646a0DOIArticle
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ORCID:
AuthorORCID
Baltimore, David0000-0001-8723-8190
Additional Information:© 1992 Nature Publishing Group. Received 21 July 1992; Accepted 05 August 1992; Issue Date 20 August 1992. We thank S. K. Burley, P. Model, X.-P. Kong, G. A. Petsko and T. P. Sakmar for suggestions. S. Nair for references to phenyl phosphate crystal structures, and B. Chait for the mass spectroscopy done at the National Resource for Mass Spectrometric Analysis of Biological Macromolecules. Supported in part by grants and fellowships from the NIH (D.B., D.C., H.H., J.K., B.J.M., C.B.R., M.D.R.), A.C.S. (M.D.R.) and Pew Foundation (J.K., M.D.R.).
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Funding AgencyGrant Number
NIHUNSPECIFIED
American Cancer SocietyUNSPECIFIED
Pew Charitable TrustUNSPECIFIED
Issue or Number:6388
Record Number:CaltechAUTHORS:20200513-153340587
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20200513-153340587
Official Citation:Waksman, G., Kominos, D., Robertson, S. et al. Crystal structure of the phosphotyrosine recognition domain SH2 of v-src complexed with tyrosine-phosphorylated peptides. Nature 358, 646–653 (1992). https://doi.org/10.1038/358646a0
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ID Code:103191
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:13 May 2020 22:52
Last Modified:13 May 2020 22:52

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