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Independent modes of transcriptional activation by the p50 and p65 subunits of NF-κB

Fujita, Takashi and Nolan, Garry P. and Ghosh, Sankar and Baltimore, David (1992) Independent modes of transcriptional activation by the p50 and p65 subunits of NF-κB. Genes and Development, 6 (5). pp. 775-787. ISSN 0890-9369. https://resolver.caltech.edu/CaltechAUTHORS:20200514-154625749

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Abstract

Recombinant subunits of the transcription factor NF-κB, p50 and p65, were analyzed both for binding to various κB motifs and in vitro activation. The subunits preferentially form a heterodimer that activates transcription. Although p50 and p65 bind DNA individually as homodimers and are structurally related, their activation mechanisms are distinct. p65 activates transcription by its unique carboxy-terminal activation domain. (p50)₂ displays higher affinity DNA binding than (p65)₂ for many distinct κB motifs and provides strong transcriptional activation only when adopting a chymotrypsin-resistant conformation induced by certain κB motifs but not others. Thus, (p50)₂ acts as a positive regulator in vitro, consistent with its isolation as a putative constitutive regulator of MHC class I genes. Both subunits of NF-κB, therefore, contribute independently to provide regulation at given κB motifs.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1101/gad.6.5.775DOIArticle
ORCID:
AuthorORCID
Baltimore, David0000-0001-8723-8190
Additional Information:© 1992 Cold Spring Harbor Laboratory Press. Received November 13, 1991; revised version accepted March 6, 1992. We thank M. Horikoshi and H. Harada for advice and help for Scatchard analysis, respectively. T.F. was supported by a fellowship from Human Frontier Science Program; G.P.N, was supported by a fellowship from National Institutes of Health (NM); S.G. was supported by a fellowship from the Irvington Institute for Medical Research. This work was supported by NIH grant GM39458-04 to D.B. The publication costs of this article were defrayed m part by payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 USC section 1734 solely to indicate this fact.
Funders:
Funding AgencyGrant Number
Human Frontier Science ProgramUNSPECIFIED
NIHGM39458-04
Irvington Institute for Medical ResearchUNSPECIFIED
Subject Keywords:NF-κB; p50; p65; c-rel; transcription; protein conformation
Issue or Number:5
Record Number:CaltechAUTHORS:20200514-154625749
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20200514-154625749
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:103216
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:14 May 2020 22:56
Last Modified:14 May 2020 22:56

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