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Independent modes of transcriptional activation by the p50 and p65 subunits of NF-κB

Fujita, Takashi and Nolan, Garry P. and Ghosh, Sankar and Baltimore, David (1992) Independent modes of transcriptional activation by the p50 and p65 subunits of NF-κB. Genes and Development, 6 (5). pp. 775-787. ISSN 0890-9369. doi:10.1101/gad.6.5.775.

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Recombinant subunits of the transcription factor NF-κB, p50 and p65, were analyzed both for binding to various κB motifs and in vitro activation. The subunits preferentially form a heterodimer that activates transcription. Although p50 and p65 bind DNA individually as homodimers and are structurally related, their activation mechanisms are distinct. p65 activates transcription by its unique carboxy-terminal activation domain. (p50)₂ displays higher affinity DNA binding than (p65)₂ for many distinct κB motifs and provides strong transcriptional activation only when adopting a chymotrypsin-resistant conformation induced by certain κB motifs but not others. Thus, (p50)₂ acts as a positive regulator in vitro, consistent with its isolation as a putative constitutive regulator of MHC class I genes. Both subunits of NF-κB, therefore, contribute independently to provide regulation at given κB motifs.

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Baltimore, David0000-0001-8723-8190
Additional Information:© 1992 Cold Spring Harbor Laboratory Press. Received November 13, 1991; revised version accepted March 6, 1992. We thank M. Horikoshi and H. Harada for advice and help for Scatchard analysis, respectively. T.F. was supported by a fellowship from Human Frontier Science Program; G.P.N, was supported by a fellowship from National Institutes of Health (NM); S.G. was supported by a fellowship from the Irvington Institute for Medical Research. This work was supported by NIH grant GM39458-04 to D.B. The publication costs of this article were defrayed m part by payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 USC section 1734 solely to indicate this fact.
Funding AgencyGrant Number
Human Frontier Science ProgramUNSPECIFIED
Irvington Institute for Medical ResearchUNSPECIFIED
Subject Keywords:NF-κB; p50; p65; c-rel; transcription; protein conformation
Issue or Number:5
Record Number:CaltechAUTHORS:20200514-154625749
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:103216
Deposited By: Tony Diaz
Deposited On:14 May 2020 22:56
Last Modified:16 Nov 2021 18:19

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