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Functional activity of myogenic HLH proteins requires hetero-oligomerization with E12/E47-like proteins in vivo

Lassar, Andrew B. and Davis, Robert L. and Wright, Woodring E. and Kadesch, Tom and Murre, Cornelis and Voronova, Anna and Baltimore, David and Weintraub, Harold (1991) Functional activity of myogenic HLH proteins requires hetero-oligomerization with E12/E47-like proteins in vivo. Cell, 66 (2). pp. 305-315. ISSN 0092-8674. doi:10.1016/0092-8674(91)90620-e. https://resolver.caltech.edu/CaltechAUTHORS:20200514-163038774

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Abstract

In this report we provide four lines of evidence indicating that E12/E47-like proteins interact in vivo with the myogenic HLH proteins MyoD and myogenin. First, cotransfection of MyoD and E47 in COS cells indicates that these factors synergistically enhance transcription of a reporter gene containing an oligomerized MyoD-binding site. Second, mobility-shift assays of muscle cell nuclear extracts, “double shifted” with specific antisera, have identified complexes binding to the MEF1 site that contain either MyoD or myogenin in association with E12/E47-like proteins. Third, association with E47 alters the phosphorylation state of MyoD. Fourth, C3H10T1/2 cells expressing antisense E2A transcripts contain low levels of E2A gene products and display less terminal muscle differentiation when infected with retroviral MyoD or when challenged to differentiate with 5-azacytidine treatment. In addition we demonstrate that MyoD, in conjunction with E12/E47-like proteins, is functioning as a regulatory nodal point for activation of several other downstream muscle regulators.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1016/0092-8674(91)90620-eDOIArticle
ORCID:
AuthorORCID
Baltimore, David0000-0001-8723-8190
Additional Information:© 1991 Published by Elsevier Inc. Received 2 November 1990, Accepted 24 May 1991. We thank Bruce Paterson for SV2-chicken MyoD, Janet Mar and Charles Ordahl for M-CAT oligonucleotides, and our colleagues at the Hutchinson Center for their thoughtful comments and suggestions. This work was supported by grants from the NIH, Howard Hughes Medical Institute, and the Lucille P. Markey Charitable Trust; a portion of this work was supported by Public Health Service grant GM39458 (D. B.). A. B. L. is a Lucille P. Markey Scholar. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 USC Section 1734 solely to indicate this fact.
Funders:
Funding AgencyGrant Number
NIHGM39458
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
Lucille P. Markey Charitable TrustUNSPECIFIED
Issue or Number:2
DOI:10.1016/0092-8674(91)90620-e
Record Number:CaltechAUTHORS:20200514-163038774
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20200514-163038774
Official Citation:Andrew B. Lassar, Robert L. Davis, Woodring E. Wright, Tom Kadesch, Cornelis Murre, Anna Voronova, David Baltimore, Harold Weintraub, Functional activity of myogenic HLH proteins requires hetero-oligomerization with E12/E47-like proteins in vivo, Cell, Volume 66, Issue 2, 1991, Pages 305-315, ISSN 0092-8674, https://doi.org/10.1016/0092-8674(91)90620-E. (http://www.sciencedirect.com/science/article/pii/009286749190620E)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:103220
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:14 May 2020 23:40
Last Modified:16 Nov 2021 18:19

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