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Helix-loop-helix transcription factor E47 activates germ-line immunoglobulin heavy-chain gene transcription and rearrangement in a pre-T-cell line

Schlissel, Mark and Voronova, Anna and Baltimore, David (1991) Helix-loop-helix transcription factor E47 activates germ-line immunoglobulin heavy-chain gene transcription and rearrangement in a pre-T-cell line. Genes and Development, 5 (8). pp. 1367-1376. ISSN 0890-9369. doi:10.1101/gad.5.8.1367.

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E47 is a helix-loop-helix transcription factor that binds to sites in the immunoglobulin heavy-chain and κ light-chain gene enhancers. Other proteins of this type are involved in cell-type determination. A possible role for E47 in B-cell development was tested by overexpressing a cDNA encoding E47 in the pre-T-cell line 2017. We found a dramatic activation of a germ-line heavy-chain gene transcript in these stable transfectants and an equally large induction of immunoglobulin D-to-J rearrangement, the first recognized step in B-cell development. Germ-line κ light-chain gene transcription and rearrangement were unaffected, but transcription of the recombination-activating genes RAG-1 and RAG-2 and the lymphoid-specific transcription factor Oct-2 was increased. These T cells did not transcribe their rearranged DJ alleles, however, and failed to progress to the next stage of heavy-chain gene assembly, V-to-DJ rearrangement. Because transcription factor E47 can induce pre-T cells to carry out events of B-cell differentiation, it may be a crucial determinant of the earliest stages of B-cell development.

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Baltimore, David0000-0001-8723-8190
Additional Information:© 1991 by Cold Spring Harbor Laboratory Press. Received April 4, 1991; revised version accepted May 14, 1991. We thank David Schatz and Lynn Corcoran for helpful comments on the manuscript and David Schatz for the gift of RAG gene PCR oligonucleotides. M.S. acknowledges the support of a Bristol-Myers Cancer Research Fellowship. A.V. was supported by fellowships from the American Cancer Society and the Cancer Research Institute. This work was supported by U.S. Public Health Service grant GM 39458. The publication costs of this article were defrayed in part by payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 USC section 1734 solely to indicate this fact.
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Bristol-Myers SquibbUNSPECIFIED
American Cancer SocietyUNSPECIFIED
Cancer Research InstituteUNSPECIFIED
Subject Keywords:Immunoglobulin genes; B cells; development; gene rearrangement
Issue or Number:8
Record Number:CaltechAUTHORS:20200515-074204649
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:103223
Deposited By: Tony Diaz
Deposited On:15 May 2020 15:17
Last Modified:16 Nov 2021 18:19

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