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DNA binding and IκB inhibition of the cloned p65 subunit of NF-κB, a rel-related polypeptide

Nolan, Garry P. and Ghosh, Sankar and Liou, Hsiou-Chi and Tempst, Paul and Baltimore, David (1991) DNA binding and IκB inhibition of the cloned p65 subunit of NF-κB, a rel-related polypeptide. Cell, 64 (5). pp. 961-969. ISSN 0092-8674. doi:10.1016/0092-8674(91)90320-x.

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The sequence and biochemical properties of the product of the cloned cDNA for the p65 subunit of nuclear factor κB (NF-κB) have been determined. The cDNA has an open reading frame of 549 amino acids capable of encoding a 60 kd protein. NF-κB p65 contains an amino-terminal region of 320 amino acids with extensive similarity to the oncogene c-rel and lesser similarity to NF-κB p50. In vitro translated p65 forms a DNA-binding complex with NF-κB p50, and the binding of this complex can be specifically inhibited by purified IκB. Progressive carboxy-terminal deletions of p65 show that, contrary to previous assumptions, p65 does include a DNA-binding domain that in vivo might become activated only through hetero-oligomerization with p50. DNA binding by truncated p65 is inhibited by IκB, thus mapping the IκB interaction domain to the rel-homologous region and suggesting that IκB exerts its inhibitory effect upon NF-κB primarily through interaction with p65.

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Baltimore, David0000-0001-8723-8190
Additional Information:© 1991 by Cell Press. Received 21 November 1990, Revised 21 December 1990. The authors are indebted to Marjorie Oetlinger, Daniel Silver, Drs. Mark Schlissel, Douglas Black, David Schatz. Rick Van Etten, and other members of the Baltimore laboratory for salient advice and gifts of reagents. We thank M. Dettinger for many helpful comments on the manuscript. We thank Ann Gifford and Lise Riviere for technical assistance. G. P. N. was supported by a fellowship from the National Institutes of Health; S. G. was supported by a fellowship from the Irvington Institute for Medical Research; H.-C. L. was supported by the Cancer Research Institute. This work was supported by NIH grant GM39458 to D. B. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement” in accordance with 18 USC Section 1734 solely to indicate this fact. GenBank Accession Number: The GenBank accession number for the sequence reported in this article is M61909.
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Irvington Institute for Medical ResearchUNSPECIFIED
Cancer Research InstituteUNSPECIFIED
Issue or Number:5
Record Number:CaltechAUTHORS:20200518-071259231
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Official Citation:Garry P. Nolan, Sankar Ghosh, Hsiou-Chi Liou, Paul Tempst, David Baltimore, DNA binding and IκB inhibition of the cloned p65 subunit of NF-κB, a rel-related polypeptide, Cell, Volume 64, Issue 5, 1991, Pages 961-969, ISSN 0092-8674, (
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:103254
Deposited By: Tony Diaz
Deposited On:18 May 2020 16:08
Last Modified:16 Nov 2021 18:19

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