CaltechAUTHORS
  A Caltech Library Service

An inhibitory domain of E12 transcription factor prevents DNA binding in E12 homodimers but not in E12 heterodimers

Sun, Xiao-Hong and Baltimore, David (1991) An inhibitory domain of E12 transcription factor prevents DNA binding in E12 homodimers but not in E12 heterodimers. Cell, 64 (2). pp. 459-470. ISSN 0092-8674. https://resolver.caltech.edu/CaltechAUTHORS:20200518-075320939

Full text is not posted in this repository. Consult Related URLs below.

Use this Persistent URL to link to this item: https://resolver.caltech.edu/CaltechAUTHORS:20200518-075320939

Abstract

The κE2 sequence binding proteins, E12 and E47, are generated by alternative splicing of the E2A gene, giving closely related basic and helix-loop-helix structures crucial for DNA binding and dimerization. Measurements of dimerization constants and binding strengths to the optimal DNA sequence (the κE2 site or its near relatives) showed that E47 homodimers and MyoD heterodimers with E12 or E47 dimerized and bound avidly, but E12 homodimerized efficiently and bound to DNA poorly; MyoD homodimerized poorly and bound strongly. An inhibitory domain N-terminal to the basic region of E12 prevents E12 homodimers but not E12/MyoD heterodimers from binding to DNA. Thus, E47 binds to DNA both as a heterodimer with MyoD and as a homodimer, while E12 and MyoD bind to DNA efficiently only as heterodimers.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1016/0092-8674(91)90653-gDOIArticle
https://doi.org/10.1016/0092-8674(81)90003-9DOICorrection
ORCID:
AuthorORCID
Baltimore, David0000-0001-8723-8190
Additional Information:© 1991 by Cell Press. Received 25 June 1990, Revised 26 September 1990. We are grateful to S. Ghosh, C. Murre, P. Jackson, and R. Andino for helpful advice and discussions. X.-H.S. was supported by a postdoctoral fellowship from the Cancer Research Institute. This work was supported by Public Health Service grant #GM39458. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 USC Section 1734 solely to indicate this fact.
Funders:
Funding AgencyGrant Number
Cancer Research InstituteUNSPECIFIED
NIHGM39458
Issue or Number:2
Record Number:CaltechAUTHORS:20200518-075320939
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20200518-075320939
Official Citation:Xiao-Hong Sun, David Baltimore, An inhibitory domain of E12 transcription factor prevents DNA binding in E12 homodimers but not in E12 heterodimers, Cell, Volume 64, Issue 2, 1991, Pages 459-470, ISSN 0092-8674, https://doi.org/10.1016/0092-8674(91)90653-G. (http://www.sciencedirect.com/science/article/pii/009286749190653G)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:103259
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:18 May 2020 15:32
Last Modified:19 May 2020 16:40

Repository Staff Only: item control page