CaltechAUTHORS
  A Caltech Library Service

Dynamically Programmable Magnetic Fields for Controlled Movement of Cells Loaded with Iron Oxide Nanoparticles

Pai, Alex and Cao, Pengpeng and White, Ethan E. and Hong, Brian and Pailevanian, Torkom and Wang, Michelle and Badie, Behnam and Hajimiri, Ali and Berlin, Jacob M. (2020) Dynamically Programmable Magnetic Fields for Controlled Movement of Cells Loaded with Iron Oxide Nanoparticles. ACS Applied Bio Materials, 3 (7). pp. 4139-4147. ISSN 2576-6422. https://resolver.caltech.edu/CaltechAUTHORS:20200528-104303291

[img] PDF - Accepted Version
See Usage Policy.

1445Kb
[img] PDF (Figure S1- Additional TEM images of loaded cells) - Supplemental Material
See Usage Policy.

5Mb
[img] Video (QuickTime) (Video S1 - Video showing the positive control for movement of magnetic particles) - Supplemental Material
See Usage Policy.

185Mb
[img] Video (AVI) (Video S2 - Video showing aggregation of neural stem cells) - Supplemental Material
See Usage Policy.

21Mb
[img] Video (AVI) (Video S3 - Video showing aggregation of THP-1 monocytes) - Supplemental Material
See Usage Policy.

33Mb
[img] Video (AVI) (Video S4 - Video showing aggregation of CAR T cells) - Supplemental Material
See Usage Policy.

35Mb
[img] Video (AVI) ((Video S5) Video showing the negative control for nonmagnetic movement of cells related to the experimental setup using loaded CAR T cells) - Supplemental Material
See Usage Policy.

67Mb

Use this Persistent URL to link to this item: https://resolver.caltech.edu/CaltechAUTHORS:20200528-104303291

Abstract

Cell-based therapies are becoming increasingly prominent in numerous medical contexts, particularly in regenerative medicine and the treatment of cancer. However, since the efficacy of the therapy is largely dependent on the concentration of therapeutic cells at the treatment area, a major challenge associated with cell-based therapies is the ability to move and localize therapeutic cells within the body. In this article, a technique based on dynamically programmable magnetic fields is successfully demonstrated to noninvasively aggregate therapeutic cells at a desired location. Various types of therapeutically relevant cells (neural stem cells, monocytes/macrophages, and chimeric antigen receptor T cells) are loaded with iron oxide nanoparticles and then focused at a particular site using externally controlled electromagnets. These experimental results serve as a readily scalable prototype for designing an apparatus that patients can wear to focus therapeutic cells at the anatomical sites needed for treatment.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1021/acsabm.0c00226DOIArticle
ORCID:
AuthorORCID
Hong, Brian0000-0001-8099-0312
Hajimiri, Ali0000-0001-6736-8019
Berlin, Jacob M.0000-0001-7498-766X
Additional Information:© 2020 American Chemical Society. Received: February 27, 2020; Accepted: May 28, 2020; Published: May 28, 2020. We gratefully acknowledge Marcia Miller, Zhuo Li, and Ricardo Zerda for electron microscopy performed in the EM core facility and Brian Armstrong, Loren Quintanar, and Tina Patel for their assistance with fluorescence imaging performed in the Light Microscopy and Digital Imaging Core. Research reported in this publication included work performed in the Electron Microscopy and the Light Microscopy and Digital Imaging Cores supported by the National Cancer Institute of the National Institutes of Health under award number P30CA033572. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. The authors would like to thank R01CA155769, R21CA189223, R21 NS081594, NIH grant 51013.914960.6692, The Kenneth T. and Eileen L. Norris Foundation, and STOP Cancer for research funding. Author Contributions: B.B., A.H., and J.M.B. contributed equally to this work. The authors declare no competing financial interest.
Funders:
Funding AgencyGrant Number
NIHP30CA033572
NIHR01CA155769
NIHR21CA189223
NIHR21NS081594
NIH51013.914960.6692
Kenneth T. and Eileen L. Norris FoundationUNSPECIFIED
STOP CancerUNSPECIFIED
Subject Keywords:CAR T cell, dynamically programmable magnetic field, immunotherapy, iron oxide nanoparticles, macrophage, magnetic transport, neural stem cell
Issue or Number:7
Record Number:CaltechAUTHORS:20200528-104303291
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20200528-104303291
Official Citation:Dynamically Programmable Magnetic Fields for Controlled Movement of Cells Loaded with Iron Oxide Nanoparticles. Alex Pai, Pengpeng Cao, Ethan E. White, Brian Hong, Torkom Pailevanian, Michelle Wang, Behnam Badie, Ali Hajimiri, and Jacob M. Berlin. ACS Applied Bio Materials 2020 3 (7), 4139-4147; DOI: 10.1021/acsabm.0c00226
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:103517
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:28 May 2020 18:38
Last Modified:31 Jul 2020 16:30

Repository Staff Only: item control page