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Ethidium analogues with improved resolution in the dye-buoyant density procedure

Hudson, Bruce and Dawson, John H. and Desiderio, Russell and Mosher, Carol W. (1977) Ethidium analogues with improved resolution in the dye-buoyant density procedure. Nucleic Acids Research, 4 (5). pp. 1349-1360. ISSN 0305-1048. PMCID PMC343760. https://resolver.caltech.edu/CaltechAUTHORS:20200608-115353955

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Abstract

Analogues of ethidium chloride with large quaternary side chains have been synthesized and evaluated for improved resolution in the dye-buoyant density system for the separation of open and closed circular DNA. These compounds are similar to propidium which differs from ethidium by the replacement of an ethyl group by a methyldiethylaminopropyl group. The new analogues contain a triethylamino group attached to a methylene chain with 3, 5 or 7 carbons. With PM2 DNA the observed separation for propidium is 1.97 times that of ethidium and the new dyes show separations relative to ethidium of 2.27, 2.66 and 2.77. A correlation is established between the mass of the dye component and the observed separation which is rationalized on the basis of the four component thermodynamics describing this system.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1093/nar/4.5.1349DOIArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/pmc343760/PubMed CentralArticle
Additional Information:© 1977 Information Retrieval Limited. Received 12 January 1977. This work was supported by a grant from the Research Corporation and U. S. Public Health Service grant CA15461. B. H. is a Camille and Henry Dreyfus Teacher-Scholar and an Alfred P. Sloan Foundation Fellow. We wish to thank Eric Rothenberg who prepared the PM2 phage.
Funders:
Funding AgencyGrant Number
Research CorporationUNSPECIFIED
NIHCA15461
Camille and Henry Dreyfus FoundationUNSPECIFIED
Alfred P. Sloan FoundationUNSPECIFIED
Issue or Number:5
PubMed Central ID:PMC343760
Record Number:CaltechAUTHORS:20200608-115353955
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20200608-115353955
Official Citation:Bruce Hudson, John H. Dawson, Russell Desiderio, Carol W. Mosher, Ethidium analogues with improved resolution in the dye-buoyant density procedure , Nucleic Acids Research, Volume 4, Issue 5, 1 June 1977, Pages 1349–1360, https://doi.org/10.1093/nar/4.5.1349
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:103762
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:08 Jun 2020 22:52
Last Modified:08 Jun 2020 22:52

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