Hudson, Bruce and Dawson, John H. and Desiderio, Russell and Mosher, Carol W. (1977) Ethidium analogues with improved resolution in the dye-buoyant density procedure. Nucleic Acids Research, 4 (5). pp. 1349-1360. ISSN 0305-1048. PMCID PMC343760. doi:10.1093/nar/4.5.1349. https://resolver.caltech.edu/CaltechAUTHORS:20200608-115353955
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Abstract
Analogues of ethidium chloride with large quaternary side chains have been synthesized and evaluated for improved resolution in the dye-buoyant density system for the separation of open and closed circular DNA. These compounds are similar to propidium which differs from ethidium by the replacement of an ethyl group by a methyldiethylaminopropyl group. The new analogues contain a triethylamino group attached to a methylene chain with 3, 5 or 7 carbons. With PM2 DNA the observed separation for propidium is 1.97 times that of ethidium and the new dyes show separations relative to ethidium of 2.27, 2.66 and 2.77. A correlation is established between the mass of the dye component and the observed separation which is rationalized on the basis of the four component thermodynamics describing this system.
| Item Type: | Article | ||||||||||
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| Additional Information: | © 1977 Information Retrieval Limited. Received 12 January 1977. This work was supported by a grant from the Research Corporation and U. S. Public Health Service grant CA15461. B. H. is a Camille and Henry Dreyfus Teacher-Scholar and an Alfred P. Sloan Foundation Fellow. We wish to thank Eric Rothenberg who prepared the PM2 phage. | ||||||||||
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| Issue or Number: | 5 | ||||||||||
| PubMed Central ID: | PMC343760 | ||||||||||
| DOI: | 10.1093/nar/4.5.1349 | ||||||||||
| Record Number: | CaltechAUTHORS:20200608-115353955 | ||||||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20200608-115353955 | ||||||||||
| Official Citation: | Bruce Hudson, John H. Dawson, Russell Desiderio, Carol W. Mosher, Ethidium analogues with improved resolution in the dye-buoyant density procedure , Nucleic Acids Research, Volume 4, Issue 5, 1 June 1977, Pages 1349–1360, https://doi.org/10.1093/nar/4.5.1349 | ||||||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | ||||||||||
| ID Code: | 103762 | ||||||||||
| Collection: | CaltechAUTHORS | ||||||||||
| Deposited By: | George Porter | ||||||||||
| Deposited On: | 08 Jun 2020 22:52 | ||||||||||
| Last Modified: | 16 Nov 2021 18:25 |
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