A Caltech Library Service

Ethidium analogues with improved resolution in the dye-buoyant density procedure

Hudson, Bruce and Dawson, John H. and Desiderio, Russell and Mosher, Carol W. (1977) Ethidium analogues with improved resolution in the dye-buoyant density procedure. Nucleic Acids Research, 4 (5). pp. 1349-1360. ISSN 0305-1048. PMCID PMC343760. doi:10.1093/nar/4.5.1349.

[img] PDF - Published Version
See Usage Policy.


Use this Persistent URL to link to this item:


Analogues of ethidium chloride with large quaternary side chains have been synthesized and evaluated for improved resolution in the dye-buoyant density system for the separation of open and closed circular DNA. These compounds are similar to propidium which differs from ethidium by the replacement of an ethyl group by a methyldiethylaminopropyl group. The new analogues contain a triethylamino group attached to a methylene chain with 3, 5 or 7 carbons. With PM2 DNA the observed separation for propidium is 1.97 times that of ethidium and the new dyes show separations relative to ethidium of 2.27, 2.66 and 2.77. A correlation is established between the mass of the dye component and the observed separation which is rationalized on the basis of the four component thermodynamics describing this system.

Item Type:Article
Related URLs:
URLURL TypeDescription CentralArticle
Additional Information:© 1977 Information Retrieval Limited. Received 12 January 1977. This work was supported by a grant from the Research Corporation and U. S. Public Health Service grant CA15461. B. H. is a Camille and Henry Dreyfus Teacher-Scholar and an Alfred P. Sloan Foundation Fellow. We wish to thank Eric Rothenberg who prepared the PM2 phage.
Funding AgencyGrant Number
Research CorporationUNSPECIFIED
Camille and Henry Dreyfus FoundationUNSPECIFIED
Alfred P. Sloan FoundationUNSPECIFIED
Issue or Number:5
PubMed Central ID:PMC343760
Record Number:CaltechAUTHORS:20200608-115353955
Persistent URL:
Official Citation:Bruce Hudson, John H. Dawson, Russell Desiderio, Carol W. Mosher, Ethidium analogues with improved resolution in the dye-buoyant density procedure , Nucleic Acids Research, Volume 4, Issue 5, 1 June 1977, Pages 1349–1360,
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:103762
Deposited By: George Porter
Deposited On:08 Jun 2020 22:52
Last Modified:16 Nov 2021 18:25

Repository Staff Only: item control page