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Early Fluorescence Signals Detect Transitions at Mammalian Serotonin Transporters

Li, Ming and Lester, Henry A. (2002) Early Fluorescence Signals Detect Transitions at Mammalian Serotonin Transporters. Biophysical Journal, 83 (1). pp. 206-218. ISSN 0006-3495. PMCID PMC1302140. doi:10.1016/s0006-3495(02)75162-x. https://resolver.caltech.edu/CaltechAUTHORS:20200612-113333889

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Abstract

The mammalian serotonin transporters rSERT or hSERT were expressed in oocytes and labeled with sulforhodamine-MTS. The endogenous Cys-109 residue contributes most of the signal, and the labeled transporter shows normal function. The SERT fluorescence decreases in the presence of 5-HT and also depends on the inorganic substrates of SERT. The fluorescence also increases with membrane depolarization. During voltage-jump experiments, fluorescence relaxations show little inactivation or history dependence. The fluorescence signal has a voltage dependence similar to that of the prepriming step of the previously described voltage-dependent transient current. However, the fluorescence relaxations are the fastest voltage-dependent events yet studied at SERT; their time constants of ∼8–30 ms are severalfold faster than the prepriming or inactivation phases of the transient currents. These fluorescence signals are interpreted within the framework of the gate-lumen-gate model. The signals may monitor initial events at the outer gate.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1016/s0006-3495(02)75162-xDOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1302140/PubMed CentralArticle
ORCID:
AuthorORCID
Lester, Henry A.0000-0002-5470-5255
Additional Information:© 2002 The Biophysical Society. Submitted September 24, 2001, and accepted for publication February 13, 2002. We thank Yanhe Tong for making the C109A mutant of hSERT. This research was supported by National Institutes of Health Grant DA-019121 and by a National Research Service Award to Ming Li.
Funders:
Funding AgencyGrant Number
NIHDA-019121
NIH Predoctoral FellowshipUNSPECIFIED
Issue or Number:1
PubMed Central ID:PMC1302140
DOI:10.1016/s0006-3495(02)75162-x
Record Number:CaltechAUTHORS:20200612-113333889
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20200612-113333889
Official Citation:Ming Li, Henry A. Lester, Early Fluorescence Signals Detect Transitions at Mammalian Serotonin Transporters, Biophysical Journal, Volume 83, Issue 1, 2002, Pages 206-218, ISSN 0006-3495, https://doi.org/10.1016/S0006-3495(02)75162-X.
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:103870
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:12 Jun 2020 19:31
Last Modified:16 Nov 2021 18:26

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