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Voltage-dependent transient currents of human and rat 5-HT transporters (SERT) are blocked by HEPES and ion channel ligands

Li, Ming and Farley, Robert A. and Lester, Henry A. (2002) Voltage-dependent transient currents of human and rat 5-HT transporters (SERT) are blocked by HEPES and ion channel ligands. FEBS Letters, 513 (2-3). pp. 247-252. ISSN 0014-5793. doi:10.1016/s0014-5793(02)02322-0. https://resolver.caltech.edu/CaltechAUTHORS:20200612-113333997

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Abstract

The hyperpolarization‐activated transient current of mammalian 5‐hydroxytryptamine transporters (SERT) expressed in Xenopus oocytes was studied. Human (h) and rat (r) SERT transient currents are blocked by HEPES with changes in the waveform kinetics, and the blockade of hSERT has use‐dependent properties. HEPES also changes the time course of the prepriming step, especially for hSERT. Transient currents at hSERT and rSERT are also blocked by spermine and spermidine in the mM range, and by fluoxetine, cocaine, QX‐314, and QX‐222 in the μM range. These pharmacological and kinetic properties of transient current blockade emphasize the similarities between the transient current and phenomena at ion channels.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1016/s0014-5793(02)02322-0DOIArticle
ORCID:
AuthorORCID
Lester, Henry A.0000-0002-5470-5255
Additional Information:© 2015 Federation of European Biochemical Societies. Received 17 December 2001; revised 17 January 2002; accepted 17 January 2002. First published online 31 January 2002. We thank Chi‐Sung Chiu and Ken Philipson for helpful discussion. This work was supported by a grant from the National Institutes of Health (DA‐09121) and by an NRSA to M.L.
Funders:
Funding AgencyGrant Number
NIHDA‐09121
NIH Predoctoral FellowshipGM08042
Subject Keywords:Ion channel; Voltage jump
Issue or Number:2-3
DOI:10.1016/s0014-5793(02)02322-0
Record Number:CaltechAUTHORS:20200612-113333997
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20200612-113333997
Official Citation:Li, Ming, Farley, Robert A and Lester, Henry A( 2002), Voltage-dependent transient currents of human and rat 5-HT transporters (SERT) are blocked by HEPES and ion channel ligands, FEBS Letters, 513, doi: 10.1016/S0014-5793(02)02322-0
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:103871
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:12 Jun 2020 19:11
Last Modified:16 Nov 2021 18:26

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