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Modulation of early growth response (EGR) transcription factor-dependent gene expression by using recombinant adenovirus

Ehrengruber, Markus U. and Muhlebach, Stephan G. and Söhrman, Sophia and Leutenegger, Christian M. and Lester, Henry A. and Davidson, Norman (2000) Modulation of early growth response (EGR) transcription factor-dependent gene expression by using recombinant adenovirus. Gene, 258 (1-2). pp. 63-69. ISSN 0378-1119. https://resolver.caltech.edu/CaltechAUTHORS:20200612-115646596

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Abstract

Early growth response (EGR) transcription factors link initial cytoplasmic events to long-term alterations of cellular gene expression and are induced by various stimuli. To test their roles in cell physiology, we constructed adenoviral recombinants encoding NGFI-A binding protein 2 (NAB2, a repressor of EGR1, EGR2, and EGR3), EGR1, NAB-insensitive EGR1(I293F) (EGR1∗), EGR2, and the NAB-binding, repressive domain 1 (R1) of EGR1. These viruses regulated EGR-dependent expression of GFP and luciferase reporter genes in heterologous expression assays. Infection of a myoblast cell line with EGR1 and EGR1∗ adenovirus induced the endogenous gene for platelet-derived growth factor A chain (PDGF-A). In addition, in neuroblastoma cells, the two novel EGR1 target genes EGR3 and NAB2 were identified by using adenoviral transfer of EGR1 and EGR1∗. Our results demonstrate that recombinant adenovirus is useful to regulate heterologous and endogenous EGR target gene expression, and suggest that EGR transcription factors can autoregulate themselves.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1016/s0378-1119(00)00445-5DOIArticle
ORCID:
AuthorORCID
Lester, Henry A.0000-0002-5470-5255
Additional Information:© 2000 Elsevier. Received 16 June 2000, Revised 15 September 2000, Accepted 28 September 2000, Available online 4 December 2000. We thank Dr Jeffrey Milbrandt and Dr John Svaren for providing A2ProLuc and the cDNAs for EGR1, EGR1∗, EGR2, NAB2, R1, and R1∗, Dr Barbara J. Wold and Dr Jeong Kyo Yoon for the sGFP cDNA, Anita Buchli for the Neuro-2a cells, Susanne Erb for the C2C12 cells, Dr Hanns Möhler and Laura Huopaniemi for supporting the TaqMan PCR, and Dr John Svaren and Dr Sondra Schlesinger for helpful discussions and comments on the manuscript. This work was supported by the Swiss National Science Foundation (grant no. 31-57′125.99 to MUE, and fellowship no. 823A-053469 to CML), the National Institute of Mental Health (grant to ND), and by the Donald E. and Delia B. Baxter Foundation (fellowship to MUE).
Funders:
Funding AgencyGrant Number
Swiss National Science Foundation (SNSF)31-57′125.99
Swiss National Science Foundation (SNSF)823A-053469
National Institute of Mental Health (NIMH)UNSPECIFIED
NIHUNSPECIFIED
Donald E. and Delia B. Baxter FoundationUNSPECIFIED
Subject Keywords:Immediate early gene; Myoblast; Neuroblastoma; NGFI-A binding protein 2 (NAB2); Quantitative PCR; Viral gene transfer
Issue or Number:1-2
Record Number:CaltechAUTHORS:20200612-115646596
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20200612-115646596
Official Citation:Markus U. Ehrengruber, Stephan G. Muhlebach, Sophia Söhrman, Christian M. Leutenegger, Henry A. Lester, Norman Davidson, Modulation of early growth response (EGR) transcription factor-dependent gene expression by using recombinant adenovirus, Gene, Volume 258, Issues 1–2, 2000, Pages 63-69, ISSN 0378-1119, https://doi.org/10.1016/S0378-1119(00)00445-5.
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:103876
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:12 Jun 2020 20:37
Last Modified:12 Jun 2020 20:37

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