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Expression of GIRK (Kir3.1/Kir3.4) channels in mouse fibroblast cells with and without β1 integrins

Ivanina, Tatiana and Neusch, Clemens and Li, Yong-Xin and Tong, Yanhe and Labarca, Cesar and Mosher, Deane F. and Lester, Henry A. (2000) Expression of GIRK (Kir3.1/Kir3.4) channels in mouse fibroblast cells with and without β1 integrins. FEBS Letters, 466 (2-3). pp. 327-332. ISSN 0014-5793. doi:10.1016/s0014-5793(99)01738-x.

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G protein‐activated K⁺ channel (GIRK) subunits possess a conserved extracellular integrin‐binding motif (RGD) and bind directly to β1 integrins. We expressed GIRK1/GIRK4 channels labeled with green fluorescent protein in fibroblast cell lines expressing or lacking β1 integrins. Neither plasma membrane localization nor agonist‐evoked GIRK currents were affected by the absence of β1 integrins or by incubation with externally applied RGD‐containing peptide. Mutation of the aspartate (D) of RGD impaired currents, GIRK glycosylation, and membrane localization, but the interaction with β1 integrins remained intact. Thus, β1 integrins are not essential for functional GIRK expression; and the GIRK‐integrin interactions involve structural elements other than the RGD motif.

Item Type:Article
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Lester, Henry A.0000-0002-5470-5255
Additional Information:© 2000 Federation of European Biochemical Societies. Received 22 November 1999. We thank Nathan Dascal for helpful discussion, Steve Potter for help with confocal microscopy, and Hai‐Rong Li for help with the oocytes. This work was supported by grants from the National Institutes of Health (GM‐29836, HL21644), the US‐Israel Binational Science Foundation, and the Deutsche Forschungsgemeinschaft.
Funding AgencyGrant Number
Binational Science Foundation (USA-Israel)UNSPECIFIED
Deutsche Forschungsgemeinschaft (DFG)UNSPECIFIED
Subject Keywords:Voltage clamp; Inward rectifier K channel; Fibroblast; Green fluorescent protein
Issue or Number:2-3
Record Number:CaltechAUTHORS:20200612-132259910
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Official Citation:Ivanina, Tatiana, Neusch, Clemens, Li, Yong-Xin, Tong, Yanhe, Labarca, Cesar, Mosher, Deane F. and Lester, Henry A.( 2000), Expression of GIRK (Kir3.1/Kir3.4) channels in mouse fibroblast cells with and without β1 integrins, FEBS Letters, 466, doi: 10.1016/S0014-5793(99)01738-X
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:103880
Deposited By: George Porter
Deposited On:12 Jun 2020 20:57
Last Modified:16 Nov 2021 18:26

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