Unger, M. and Kartalov, E. and Chiu, C.-S. and Lester, H. A. and Quake, S. R. (1999) Single-Molecule Fluorescence Observed with Mercury Lamp Illumination. BioTechniques, 27 (5). pp. 1008-1014. ISSN 0736-6205. doi:10.2144/99275rr01. https://resolver.caltech.edu/CaltechAUTHORS:20200612-132300221
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Abstract
We demonstrate that it is possible to observe single fluorescent molecules using a standard fluorescence microscope with mercury lamp excitation and an inexpensive cooled charge-coupled device (CCD) camera. With this equipment, we have been able to observe single molecules of tetramethyl-rhodamine, rhodamine 6G, fluorescein isothiocyanate and green fluorescent protein. Immobilized molecules were observed both in air and in aqueous solution.
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| Additional Information: | © 1999 Future Science Group. Received 11 February 1999; accepted 13 July 1999. The S65T, V163A, I167T, S175G GFP mutant was kindly provided by Dr. Kurt Beam, Department of Anatomy and Neurobiology, Colorado State University, Fort Collins, CO, USA. This work was partially supported by the National Institutes of Health Grant Nos. NS-11756 and DA-9121. | ||||||
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| Issue or Number: | 5 | ||||||
| DOI: | 10.2144/99275rr01 | ||||||
| Record Number: | CaltechAUTHORS:20200612-132300221 | ||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20200612-132300221 | ||||||
| Official Citation: | Single-Molecule Fluorescence Observed with Mercury Lamp Illumination M. Unger, E. Kartalov, C.-S. Chiu, H.A. Lester, and S.R. Quake BioTechniques 1999 27:5, 1008-1014 | ||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | ||||||
| ID Code: | 103881 | ||||||
| Collection: | CaltechAUTHORS | ||||||
| Deposited By: | George Porter | ||||||
| Deposited On: | 12 Jun 2020 20:52 | ||||||
| Last Modified: | 16 Nov 2021 18:26 |
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