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Selective expression of RAG-2 in chicken B cells undergoing immunoglobulin gene conversion

Carlson, Louise M. and Oettinger, Marjorie A. and Schatz, David G. and Masteller, Emma L. and Hurley, Elizabeth A. and McCormack, Wayne T. and Baltimore, David and Thompson, Craig B. (1991) Selective expression of RAG-2 in chicken B cells undergoing immunoglobulin gene conversion. Cell, 64 (1). pp. 201-208. ISSN 0092-8674. doi:10.1016/0092-8674(91)90221-j. https://resolver.caltech.edu/CaltechAUTHORS:20200612-135406177

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Abstract

Chickens create their immunoglobulin (Ig) repertoires during B cell development in the bursa of Fabriclus by intrachromosomal gene conversion. Recent evidence has suggested that Ig gene conversion may involve cis-acting DNA elements related to those involved in V(D)J recombination. Therefore, we have examined the potential role of the V(D)J recombination activating genes, RAG-1 and RAG-2, in regulating chicken Ig gene conversion. In contrast to the coexpression of RAG-1 and RAG-2 observed in mammalian B cells that undergo V(D)J recombination, chicken B cells isolated from the bursa of Fabricius express high levels of the RAG-2 mRNA but do not express RAG-1 mRNA. The developmental and phenotypic characteristics of the bursal lymphocytes and chicken B cell lines that express RAG-2 mRNA demonstrate that selective RAG-2 expression occurs specifically in B cells undergoing Ig diversification by gene conversion. These data suggest that RAG-2 plays a fundamental role in Ig-specific gene conversion.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1016/0092-8674(91)90221-jDOIArticle
ORCID:
AuthorORCID
Schatz, David G.0000-0002-5669-1176
Baltimore, David0000-0001-8723-8190
Additional Information:© 1991 Cell Press. Received 2 November 1990, Revised 20 November 1990. We thank our colleagues Jeff Leiden, Eric Humphries, and John Lowe for helpful discussions and review of the manuscript, and Beverly Burck for preparation of figures. This work was supported by Public Service Award CA48023 to C. B. T., and CA51466 to D. B. from the National Institutes of Health, and by fellowships from the Whitaker Health Sciences Fund (M. A. O.) and the Life and Health Insurance Medical Research Fund (D. G. S.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked “advertisement” in accordance with 18 USC Section 1734 solely to indicate this fact. GenBank Accession Numbers: The accession numbers for the sequences reported in this paper are M58530 (chicken RAG-1) and M58531 (chicken RAG-2).
Funders:
Funding AgencyGrant Number
NIH Predoctoral FellowshipCA48023
NIHCA51466
Whitaker Health Science FundUNSPECIFIED
Life and Health Insurance Medical Research FundUNSPECIFIED
Issue or Number:1
DOI:10.1016/0092-8674(91)90221-j
Record Number:CaltechAUTHORS:20200612-135406177
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20200612-135406177
Official Citation:Louise M. Carlson, Marjorie A. Oettinger, David G. Schatz, Emma L. Masteller, Elizabeth A. Hurley, Wayne T. McCormack, David Baltimore, Craig B. Thompson, Selective expression of RAG-2 in chicken B cells undergoing immunoglobulin gene conversion, Cell, Volume 64, Issue 1, 1991, Pages 201-208, ISSN 0092-8674, https://doi.org/10.1016/0092-8674(91)90221-J.
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:103885
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:12 Jun 2020 21:27
Last Modified:16 Nov 2021 18:26

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