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The recombination activating gene-1 (RAG-1) transcript is present in the murine central nervous system

Chun, Jerold J. M. and Schatz, David G. and Oettinger, Marjorie A. and Jaenisch, Rudolf and Baltimore, David (1991) The recombination activating gene-1 (RAG-1) transcript is present in the murine central nervous system. Cell, 64 (1). pp. 189-200. ISSN 0092-8674. doi:10.1016/0092-8674(91)90220-s.

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The recombination activating genes, RAG-1 and RAG-2, are likely to encode components of the V(D)J site-specific recombination machinery. We report here the detection of low levels of the RAG-1 transcrlpt in the murlne central nervous system by polymerase chain reaction, In situ hybridization, and Northern blot analyses. In contrast, an authentic RAG-2 transcript could not be detected reproduclbly in the central nervous system. The RAG-1 transcript was found to be wide-spread in embryonic and postnatal neurons, with transcription being most apparent in regions of the postnatal brain with a high neuronal cell denslty (the cerebellum and the hippocampal formation). The results suggest that RAG-1 functions in neurons, where its role might be to recombine elements of the neuronal genome site-specifically, or to prevent detrimental alterations of the genome in these long-lived cells.

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Schatz, David G.0000-0002-5669-1176
Baltimore, David0000-0001-8723-8190
Additional Information:© 1991 Cell Press. Received 2 November 1990. We are deeply indebted to Carolyn Gorka for her superb technical assistance in the synthesis of the riboprobes used in these experiments and in numerous other aspects of the work. We thank Doug Black for RNA samples for the early analysis of differentiated P19 cells. We thank Doug Black, Barbara Ruskin, Dan Silver, and Craig Thompson for critical reading of and many helpful comments on the manuscript. This work was supported by NIH grants (OIG) 5R35-CA 44339 to R. J. and CA51466 to D. B. and by fellowships from the Helen Hay Whitney Foundation (J. J. M. C.), the Life and Health Insurance Medical Research Fund (D. G. S.), and the Whitaker Health Sciences Fund (M. A. O.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked “advertisement” in accordance with 16 USC Section 1734 solely to indicate this fact.
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NIH5R35-CA 44339
Helen Hay Whitney FoundationUNSPECIFIED
Life and Health Insurance Medical Research FundUNSPECIFIED
Whitaker Health Science FundUNSPECIFIED
Issue or Number:1
Record Number:CaltechAUTHORS:20200612-135406275
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Official Citation:Jerold J.M. Chun, David G. Schatz, Marjorie A. Oettinger, Rudolf Jaenisch, David Baltimore, The recombination activating gene-1 (RAG-1) transcript is present in the murine central nervous system, Cell, Volume 64, Issue 1, 1991, Pages 189-200, ISSN 0092-8674,
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:103886
Deposited By: George Porter
Deposited On:12 Jun 2020 21:24
Last Modified:16 Nov 2021 18:26

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