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A functional ribonucleoprotein complex forms around the 5′ end of poliovirus RNA

Andino, Raul and Rieckhof, Gabrielle E. and Baltimore, David (1990) A functional ribonucleoprotein complex forms around the 5′ end of poliovirus RNA. Cell, 63 (2). pp. 369-380. ISSN 0092-8674. https://resolver.caltech.edu/CaltechAUTHORS:20200612-135406435

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Abstract

The existence of a computer-predicted cloverleaf structure for the first 100 nucleotides at the 5′ end of poliovirus RNA was verified by site-directed mutagenesis and by chemical and RNAase probing. Mutations that modified the cloverleaf in the positive strand but not the negative strand were lethal to the virus. This RNA cloverleaf structure binds a cellular protein and the viral proteins 3Cᵖʳᵒ and 3Dᵖᵒⁱ. Mutations in specific regions of the RNA cloverleaf prevented this binding. Mutations in either 3Cᵖʳᵒ or the RNA that disrupted ribonucleoprotein complex formation inhibited virus growth and selectively affected positive strand RNA accumulation. Phenotypic reversion of these mutations restored the ability to form the complex. Thus, a cloverleaf structure in poliovirus RNA plays a central role in organizing viral and cellular proteins involved in positive strand production.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1016/0092-8674(90)90170-jDOIArticle
ORCID:
AuthorORCID
Baltimore, David0000-0001-8723-8190
Additional Information:© 1990 Cell Press. Received 16 May 1990, Revised 2 August 1990. We are grateful to Drs. Nahum Sonenberg and Douglas Black for technical advice, Cristina Giachetti for anti-3C antiserum, Gerardo Kaplan and Cara Burns for anti-3D antiserum, and Douglas Black, Xiao-Hong Sun, Mark Muesing, and Mark Feinberg for their useful comments on the manuscript. This work was supported by Public Health Service grant #AI 22346 to D. B. R. A. is recipient of a fellowship from the Consejo National de Investigaciones Cientificas y Tecnicas-Argentina. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
Funders:
Funding AgencyGrant Number
NIHAI 22346
Consejo National de Investigaciones Cientificas y Tecnicas (Argentina)UNSPECIFIED
Issue or Number:2
Record Number:CaltechAUTHORS:20200612-135406435
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20200612-135406435
Official Citation:Raul Andino, Gabrielle E. Rieckhof, David Baltimore, A functional ribonucleoprotein complex forms around the 5′ end of poliovirus RNA, Cell, Volume 63, Issue 2, 1990, Pages 369-380, ISSN 0092-8674, https://doi.org/10.1016/0092-8674(90)90170-J.
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:103888
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:12 Jun 2020 21:48
Last Modified:12 Jun 2020 21:48

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