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Cloning of the p50 DNA binding subunit of NF-κB: Homology to rel and dorsal

Ghosh, Sankar and Gifford, Ann M. and Riviere, Lise R. and Tempst, Paul and Nolan, Garry P. and Baltimore, David (1990) Cloning of the p50 DNA binding subunit of NF-κB: Homology to rel and dorsal. Cell, 62 (5). pp. 1019-1029. ISSN 0092-8674. https://resolver.caltech.edu/CaltechAUTHORS:20200612-145542160

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Abstract

The DNA binding subunit of the transcription factor NF-κB, p50, has been cloned. p50 appears to be synthesized as a larger protein that is then processed to its functional size. Sequence analysis reveals remarkable homology for over 300 amino acids at the amino-terminal end to the oncogene v-rel, its cellular homolog c-rel, and the Drosophila maternal effect gene dorsal. This establishes NF-κB as a member of the rel family of proteins, all of which display nuclear-cytosolic translocation. Protein sequence from the p65 polypeptide has established that it is not encoded in the same mRNA as p50. However, p65 appears homologous to c-rel, suggesting that c-rel may form heterodimers with p50 and rel may include a homodimerization motif.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1016/0092-8674(90)90276-kDOIArticle
ORCID:
AuthorORCID
Baltimore, David0000-0001-8723-8190
Additional Information:© 1990 Cell Press. Received 10 July 1990, Revised 30 July 1990. We would like to thank Dr. Atain Israēll for sharing the sequence for the human p50 NF-κB/KBF-1 with s before publication, and the reviewers for their careful analysis of our manuscript. We would also like to thank Marjorie Cettinger, Dr. David G. Schatz, and Dr. Mark Schlissel for their material and intellectual help; Dm. X.-H. Sun, D. Black, and M. Feinberg for helpful advise and discussions; and M. Paskind and Dr. R. A. VanEtten for help with photography. This work was supported by Public Health Service grant GM39458 (to D. B.). S. G. was supported by a postdoctoral fellowship from the Irvington institute for Medical Research, and G.P N. was supported by a fellowship from the National Institutes of Health. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked “advertisement” in accordance with 18 USC. Section 1734 solely to indicate this fact.
Funders:
Funding AgencyGrant Number
NIHGM39458
Irvington Institute for Medical ResearchUNSPECIFIED
NIH Postdoctoral FellowshipUNSPECIFIED
Issue or Number:5
Record Number:CaltechAUTHORS:20200612-145542160
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20200612-145542160
Official Citation:Sankar Ghosh, Ann M. Gifford, Lise R. Riviere, Paul Tempst, Garry P. Nolan, David Baltimore, Cloning of the p50 DNA binding subunit of NF-κB: Homology to rel and dorsal, Cell, Volume 62, Issue 5, 1990, Pages 1019-1029, ISSN 0092-8674, https://doi.org/10.1016/0092-8674(90)90276-K.
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:103892
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:15 Jun 2020 15:20
Last Modified:15 Jun 2020 15:20

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