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Cells nonproductively infected with HIV-1 exhibit an aberrant pattern of viral RNA expression: A molecular model for latency

Pomerantz, Roger J. and Trono, Didier and Feinberg, Mark B. and Baltimore, David (1990) Cells nonproductively infected with HIV-1 exhibit an aberrant pattern of viral RNA expression: A molecular model for latency. Cell, 61 (7). pp. 1271-1276. ISSN 0092-8674. doi:10.1016/0092-8674(90)90691-7. https://resolver.caltech.edu/CaltechAUTHORS:20200612-145542290

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Abstract

U1 and ACH-2 cells are subclones of HIV-1-infected monocyte/macrophage-like and T lymphocyte cell lines, respectively, which express the HIV-1 genome at very low levels. We have examined whether they might provide a model of HIV-1 latency. The patterns of HIV-1-specific RNA expressed in these cells consisted of singly and multiply spliced RNA species, with little or no full-length genomic RNA. Upon stimulation with agents that activate the HIV-1 long terminal repeat in these cells, a marked rise in the amount of small mRNAs, encoding the viral regulatory proteins, preceded the increase in the unspliced RNA. Thus, U1 and ACH-2 cells maintain HIV-1 in a state equivalent to the early phase of a lytic infection and, after stimulation, recapitulate the events of a single cycle infection of highly susceptible target cells.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1016/0092-8674(90)90691-7DOIArticle
ORCID:
AuthorORCID
Baltimore, David0000-0001-8723-8190
Additional Information:© 1990 Cell Press. Received 2 April 1990, Revised 19 April 1990. The authors would like to thank Dr. Anthony Fauci, whose discussions of these cell lines motivated the present studies. We would also like to thank Drs. Mark Mussing and Daniel Chang for critical discussions and Nancy Kong for assistance with the densitometer. R. J. P. was funded by Physician Scientist Award #A100930, and this work was in part funded by Public Health Service grants AI26463 and HL43510. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked “advertisement” in accordance with 16 U.S.C. Section 1734 solely to indicate this fact.
Funders:
Funding AgencyGrant Number
NIHAI00930
NIHAI26463
NIHHL43510
Issue or Number:7
DOI:10.1016/0092-8674(90)90691-7
Record Number:CaltechAUTHORS:20200612-145542290
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20200612-145542290
Official Citation:Roger J. Pomerantz, Didier Trono, Mark B. Feinberg, David Baltimore, Cells nonproductively infected with HIV-1 exhibit an aberrant pattern of viral RNA expression: A molecular model for latency, Cell, Volume 61, Issue 7, 1990, Pages 1271-1276, ISSN 0092-8674, https://doi.org/10.1016/0092-8674(90)90691-7.
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:103893
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:15 Jun 2020 15:38
Last Modified:16 Nov 2021 18:26

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