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DVC interneuron cGAL driver in Caenorhabditis elegans

Oh, Jun Young and Gharib, Shahla and Liu, Jonathan and Wang, Han and Sternberg, Paul W. (2019) DVC interneuron cGAL driver in Caenorhabditis elegans. microPublication Biology . micropub.biology.000082. ISSN 2578-9430. PMCID PMC7255773. https://resolver.caltech.edu/CaltechAUTHORS:20200624-132515714

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Abstract

cGAL, a recently developed temperature-robust bipartite GAL4-UAS system in C. elegans, consists of two components: a cGAL “driver” that expresses the cGAL protein in specific cells using a promoter (i.e. neuron-specific or tissue-specific), and an “effector” that carries a gene of interest downstream of UAS (Wang et al., 2017). Crossing or combining a driver with an effector leads to the expression of the gene of interest in a cell-specific or tissue-specific manner. Here we report a new cGAL driver for the DVC interneuron. The ceh-63 promoter was chosen due to its restricted expression in the DVC neuron (Feng et al. 2012). The DVC interneuron driver construct containing the ceh-63 promoter (646 bp upstream of ATG translation start site) was injected into N2 and an integrated DVC driver line was generated. When crossed with the UAS-GFP effector strain (PS6843), the ceh-63 cGAL driver dictated GFP expression in the single DVC neuron (Figure 1), in addition to GFP in the coelomocyte from Punc-122::gfp co-injection marker. We did not observe GFP expression in uterus as reported by Feng et al., 2012.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.17912/micropub.biology.000082DOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7255773PubMed CentralArticle
ORCID:
AuthorORCID
Oh, Jun Young0000-0002-5158-9017
Wang, Han0000-0002-1933-5762
Sternberg, Paul W.0000-0002-7699-0173
Additional Information:© 2019 by the authors. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International (CC BY 4.0) License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Received: December 12, 2018; Accepted: February 26, 2019; Published: March 6, 2019. Funding: R21 MH115454/MH/NIMH NIH HHS/United States. Author Contributions: Jun Young Oh: Conceptualization, Methodology, Data curation, Investigation, Validation, Writing - original draft Shahla Gharib: Investigation Jonathan Liu: Conceptualization, Methodology, Supervision, Writing - review and editing Han Wang: Conceptualization, Methodology, Supervision, Writing - review and editing Paul Sternberg: Conceptualization, Methodology, Funding acquisition, Project administration, Supervision, Writing - review and editing. Reviewed By: Ian Hope.
Funders:
Funding AgencyGrant Number
NIHR21 MH115454
National Institute of Mental Health (NIMH)UNSPECIFIED
PubMed Central ID:PMC7255773
Record Number:CaltechAUTHORS:20200624-132515714
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20200624-132515714
Official Citation:Oh, JY; Gharib, S; Liu, J; Wang, H; Sternberg, P (2019). DVC interneuron cGAL driver in Caenorhabditis elegans. microPublication Biology. 10.17912/micropub.biology.000082
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:104015
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:24 Jun 2020 20:42
Last Modified:24 Jun 2020 20:42

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