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Genome editing of Caenorhabditis briggsae using CRISPR/Cas9 co-conversion marker dpy-10

Cohen, Sarah M. and Sternberg, Paul W. (2019) Genome editing of Caenorhabditis briggsae using CRISPR/Cas9 co-conversion marker dpy-10. microPublication Biology . micropub.biology.000171. ISSN 2578-9430. PMCID PMC7252229. https://resolver.caltech.edu/CaltechAUTHORS:20200625-134930761

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Abstract

Genomic editing of the roundworm Caenorhabditis elegans using the CRISPR/Cas9 system has allowed for widespread creation of null mutants – vital for scientific understanding of this model organism. A closely-related nematode species, Caenorhabditis briggsae, is emerging as an alternative model organism to better understand how findings in C. elegans can broaden and develop the larger field of nematology (Gupta et al. 2007). To that end, we have developed an effective and efficient co-conversion CRISPR/Cas9 system for use in C. briggsae using the gene dpy-10. We modified the universal STOP-IN cassette method, as described by Wang et al. (2018), for use in C. briggsae (Wang et al. 2018). Using the wildtype AF16 strain, we tested the method by choosing the gene Cbr-dpy-10 because of its readily observed predicted phenotype. Cbr-dpy-10 is a predicted one-to-one ortholog of C. elegans dpy-10, which encodes a protein important for cuticle development and has a phenotype characterized by short, fat animals relative to wild type (Brenner 1974). A universal STOP-IN allele of Cbr-dpy-10, sy1387, was generated and confirmed by genotype sequencing. The expected phenotype was subsequently observed, consistent with the creation of a null allele (Figure 1). Surprisingly, sy1387 is dominant. Injections of 20 animals produced 15 successful injections; 247 animals from these 15 injections (F1) were singled out and allowed to self-fertilize to produce F2. 81% of the F1 Dumpy progeny were homozygous as evidenced by their segregation of only Dumpy progeny – one of these candidates became sy1387. 2% of the F1s did not produce progeny. 17% of the 247 F1 Dumpy progeny were heterozygotes and had a mixture of Dumpy and non-Dumpy progeny; these non-Dumpy F2s only produced non-Dumpy progeny. This discovery of a dominant mutation will allow for more effective use of this as a co-conversion marker when screening for other mutations. We used Cbr-dpy-10 as a potential co-CRISPR marker for a second target that will be described elsewhere. We used PCR to detect insertion of a STOP-IN cassette at this other locus; we screened 39 Dumpy strains to obtain 11 candidates for our target gene, from which we have three STOP-IN alleles.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.17912/micropub.biology.000171DOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7252229PubMed CentralArticle
ORCID:
AuthorORCID
Cohen, Sarah M.0000-0002-5233-2280
Sternberg, Paul W.0000-0002-7699-0173
Additional Information:© 2019 by the authors. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International (CC BY 4.0) License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Received: September 12, 2019; Accepted: September 30, 2019; Published: October 11, 2019. We thank Heenam Park for her discussion and comments. Funding: This material is based upon work supported by the National Science Foundation Graduate Research Fellowship under Grant No. DGE 1745301 and the NIH under Grant No. R24 0D023041. Author Contributions: Sarah M Cohen: Conceptualization, Investigation, Methodology, Visualization, Writing - original draft, Writing - review and editing Paul W Sternberg: Conceptualization, Funding acquisition, Supervision, Writing - review and editing.
Funders:
Funding AgencyGrant Number
NSF Graduate Research FellowshipDGE-1745301
NIHR24 0D023041
PubMed Central ID:PMC7252229
Record Number:CaltechAUTHORS:20200625-134930761
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20200625-134930761
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:104064
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:25 Jun 2020 21:11
Last Modified:25 Jun 2020 21:11

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