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Single-channel kinetics of the rat olfactory cyclic nucleotide-gated channel expressed in Xenopus oocytes

Li, Jun and Lester, Henry A. (1999) Single-channel kinetics of the rat olfactory cyclic nucleotide-gated channel expressed in Xenopus oocytes. Molecular Pharmacology, 55 (5). pp. 883-893. ISSN 0026-895X.

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Cyclic nucleotide-gated channels are nonselective cation channels activated by intracellular cAMP and/or cGMP. It is not known how the binding of agonists opens the channel, or how the presumed four binding sites, one on each subunit, interact to generate cooperativity. We expressed the rat olfactory cyclic nucleotide-gated channel alpha subunit in Xenopus oocytes and recorded the single-channel currents. The channel had a single conductance state, and flickers at -60 mV showed the same power spectrum for cAMP and cGMP. At steady state, the distribution patterns of open and closed times were relatively simple, containing one or two exponential components. The conductance properties and the dwell-time distributions were adequately described by models that invoke only one or two binding events to open the channel, followed by an additional binding event that prolongs the openings and helps to explain apparent cooperativity. In a comparison between cAMP and cGMP, we find that cGMP has clearly higher binding affinity than cAMP, but only modestly higher probability of inducing the conformational transition that opens the channel.

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Lester, Henry A.0000-0002-5470-5255
Additional Information:© 1999 by the American Society for Pharmacology and Experimental Therapeutics. Received September 24, 1998; accepted January 28, 1999. This research was supported by a grant from the National Institutes of Health (NS-11756). We thank Yinong Zhang for much technical assistance and helpful discussions, Hairong Li and Brad Henkle for preparing oocytes, and Ben Edelman (Harvard College) for help using the MIL programs. We thank Dr. William Zagotta for insightful discussions during this project.
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Issue or Number:5
Record Number:CaltechAUTHORS:20200626-123656741
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Official Citation:Single-Channel Kinetics of the Rat Olfactory Cyclic Nucleotide-Gated Channel Expressed in Xenopus Oocytes Jun Li and Henry A. Lester Molecular Pharmacology May 1, 1999, 55 (5) 883-893
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:104082
Deposited By: George Porter
Deposited On:26 Jun 2020 22:21
Last Modified:26 Jun 2020 22:21

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