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RAG-1 and RAG-2, adjacent genes that synergistically activate V(D)J recombination

Oettinger, Marjorie A. and Schatz, David G. and Gorka, Carolyn and Baltimore, David (1990) RAG-1 and RAG-2, adjacent genes that synergistically activate V(D)J recombination. Science, 248 (4962). pp. 1517-1523. ISSN 0036-8075. doi:10.1126/science.2360047. https://resolver.caltech.edu/CaltechAUTHORS:20200626-123754898

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Abstract

The vast repertoire of immunoglobulins and T cell receptors is generated, in part, by V(D)J recombination, a series of genomic rearrangements that occur specifically in developing lymphocytes. The recombination activating gene, RAG-1, which is a gene expressed exclusively in maturing lymphoid cells, was previously isolated. RAG-1 inefficiently induced V(D)J recombinase activity when transfected into fibroblasts, but cotransfection with an adjacent gene, RAG-2, has resulted in at least a 1000-fold increase in the frequency of recombination. The 2.1-kilobase RAG-2 complementary DNA encodes a putative protein of 527 amino acids whose sequence is unrelated to that of RAG-1. Like RAG-1, RAG-2 is conserved between species that carry out V(D)J recombination, and its expression pattern correlates precisely with that of V(D)J recombinase activity. In addition to being located just 8 kilobases apart, these convergently transcribed genes are unusual in that most, if not all, of their coding and 3' untranslated sequences are contained in single exons. RAG-1 and RAG-2 might activate the expression of the V(D)J recombinase but, more likely, they directly participate in the recombination reaction.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1126/science.2360047DOIArticle
ORCID:
AuthorORCID
Schatz, David G.0000-0002-5669-1176
Baltimore, David0000-0001-8723-8190
Additional Information:© 1990 American Association for the Advancement of Science. 30 April 1990; accepted 23 May 1990. We thank R. Mosher, J. Bogan, and D. Page for the DNA's used in the Southern blot, K. Struhl for many helpful discussions, and K. Struhl, D. Black, and S. Smale for critical reading of the manuscript. Supported by NIH grant GM39458 and by fellowships from the Whitaker Health Sciences Fund (M.A.O.), and the Life and Health Insurance Medical Research Fund (O.G.S.). The GenBank accession number for the mouse RAG-2 sequence reported in this article is M33828.
Funders:
Funding AgencyGrant Number
NIHGM39458
Whitaker Health Sciences FundUNSPECIFIED
Issue or Number:4962
DOI:10.1126/science.2360047
Record Number:CaltechAUTHORS:20200626-123754898
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20200626-123754898
Official Citation:RAG-1 and RAG-2, adjacent genes that synergistically activate V(D)J recombination BY MA OETTINGER, DG SCHATZ, C GORKA, D BALTIMORE Science 22 Jun 1990: Vol. 248, Issue 4962, pp. 1517-1523 DOI: 10.1126/science.2360047
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:104089
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:26 Jun 2020 20:59
Last Modified:16 Nov 2021 18:28

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