CaltechAUTHORS
  A Caltech Library Service

Activation of interleukin-6 gene expression through the NF-κB transcription factor

Libermann, Towia A. and Baltimore, David (1990) Activation of interleukin-6 gene expression through the NF-κB transcription factor. Molecular and Cellular Biology, 10 (5). pp. 2327-2334. ISSN 0270-7306. PMCID PMC360580. doi:10.1128/mcb.10.5.2327. https://resolver.caltech.edu/CaltechAUTHORS:20200626-135308824

Full text is not posted in this repository. Consult Related URLs below.

Use this Persistent URL to link to this item: https://resolver.caltech.edu/CaltechAUTHORS:20200626-135308824

Abstract

The promoter region of the interleukin-6 (IL-6) gene has a putative NF-κB-binding site. We found that a fragment of the IL-6 promoter containing the site specifically binds highly purified NF-κB protein and the NF-κB protein in nuclear extracts of phorbol ester-induced Jurkat cells. Mutations of the NF-κB site abolished complex formation with both purified NF-κB and the nuclear extract protein. Transient expression of chloramphenicol acetyltransferase (CAT) plasmids containing the IL-6 promoter revealed very little activity of the promoter in U-937 monocytic cells and in HeLa cells before stimulation. However, stimulation of U-937 and HeLa cells by inducers of NF-κB led to a dramatic increase in CAT activity. Mutations in the NF-κB-binding site abolished inducibility of IL-6 promoter-cat constructs in U-937 cells by lipopolysaccharide, tumor necrosis factor α, the double-stranded RNA poly(IC), or phytohemagglutinin and in HeLa cells by tumor necrosis factor alpha and drastically reduced but did not completely eliminate inducibility in HeLa cells stimulated by double-stranded RNA poly(IC) or phorbol 12-myristate 13-acetate. These results suggest that NF-κB is an important mediator for activation of the IL-6 gene by a variety of IL-6 inducers in both U-937 and HeLa cells and that alternative inducible enhancer elements contribute in a cell-specific manner to IL-6 gene induction. Because NF-κB is involved in the control of a variety of genes activated upon inflammation, NF-κB may play a central role in the inflammatory response to infection and tissue injury.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1128/mcb.10.5.2327DOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC360580/PubMed CentralArticle
ORCID:
AuthorORCID
Baltimore, David0000-0001-8723-8190
Alternate Title:Activation of interleukin-6 gene expression through the NF-kappa B transcription factor
Additional Information:© 1990 American Society for Microbiology. Received 11 December 1989/Accepted 31 January 1990 We thank Patrick Baeuerle for helpful discussions and the kind gift of affinity-purified NF-κB. This work was supported by a fellowship from the Leukemia Society of America (T.A.L.) and Public Health Service grant GM 39458 to D.B. from the National Institutes of Health.
Funders:
Funding AgencyGrant Number
Leukemia Society of AmericaUNSPECIFIED
NIHGM 39458
Issue or Number:5
PubMed Central ID:PMC360580
DOI:10.1128/mcb.10.5.2327
Record Number:CaltechAUTHORS:20200626-135308824
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20200626-135308824
Official Citation:Activation of interleukin-6 gene expression through the NF-kappa B transcription factor. T A Libermann, D Baltimore Molecular and Cellular Biology May 1990, 10 (5) 2327-2334; DOI: 10.1128/MCB.10.5.2327
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:104096
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:26 Jun 2020 21:27
Last Modified:16 Nov 2021 18:28

Repository Staff Only: item control page