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Laminar organization of acetylcholinesterase and cytochrome oxidase in the lateral geniculate nucleus of prosimians

McDonald, C. T. and McGuinness, E. R. and Allman, J. M. (1993) Laminar organization of acetylcholinesterase and cytochrome oxidase in the lateral geniculate nucleus of prosimians. Neuroscience, 54 (4). pp. 1091-1101. ISSN 0306-4522. https://resolver.caltech.edu/CaltechAUTHORS:20200629-134857909

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Abstract

Hess and Rockland [Hess and Rockland (1983) Brain Res.289, 322–325] proposed that the distribution of acetylcholinesterase within the lateral geniculate nucleus might correlate with the daily activity patterns shown by primates. In diurnal primates, the magnocellular laminae show a greater acetylcholinesterase reaction product. In nocturnal primates, the parvocellular laminae are more heavily stained. We have examined the laminar distribution of acetylcholinesterase and cytochrome oxidase in the lateral geniculate nucleus of a series of rare prosimian primates. In all prosimians examined, the most dense acetylcholinesterase reaction product is seen in the parvocellular layers of the lateral geniculate nucleus. Heavy cytochrome oxidase activity is seen in both the magnocellular and parvocellular layers, but not the koniocellular layers of the prosimian lateral geniculate nucleus. We have also employed a polyclonal antibody to choline acetyltransferase to examine the laminar organization or cholinergic activity in the Galago (Bushbaby) lateral geniculate nucleus. We report that choline acetyltransferase immunoreactivity does not correlate with acetylcholinesterase activity in the prosimian lateral geniculate nucleus. Although the lateral geniculate nucleus is more immunoreactive than most other thalamic structures and although the intercalated koniocellular laminae demonstrate somewhat lighter choline acetyltransferase immunoreactivity, no great difference in staining intensity is seen between the parvocellular and magnocellular laminae. In addition, we examined the phenotype of known inputs to assess the laminar specificity of cholinergic projections to the bushbaby lateral geniculate nucleus. Layer VI of primary visual cortex, which is known to be a source of acetylcholinesterase in the parvocellular layers, does not contain cholinergic cells, nor does the pretectal nucleus, which projects mainly to the parvocellular layers. The parabigeminal nucleus is cholinergic; however, this nucleus is known to project to the koniocellular layers, along with the noncholinergic superior colliculus. Finally, the pedunculopontine tegmental nucleus, which provides a strong input to many regions of the thalamus, including the lateral geniculate nucleus, is cholinergic. The laminar organization of its input to the lateral geniculate nucleus is not known. Increased acetylcholinesterase reaction product within the parvocellular layers of the lateral geniculate nucleus is common to all strepsirhine primates. The pattern is also seen in the only two nocturnal haplorhine primates, Tarsius and Aotus (owl monkey). The relation of this increased acetylcholinesterase activity to cholinergic function remains unclear. We note exceptions to the suggested correlation between nocturnality and increased density of acetylcholinesterase within the parvocellular layers. We hypothesize that rather than reflecting nocturnal/diurnal lifestyles, the acetylcholinesterase staining pattern may be a primitive feature of geniculate organization in primates.


Item Type:Article
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https://doi.org/10.1016/0306-4522(93)90598-aDOIArticle
Additional Information:© 1993 Published by Elsevier. Accepted 9 December 1992. We are indebted to Drs Elwyn Simons and Patricia Wright of the Duke University Primate Center for providing the rare prosimian brains used in this study. We also gratefully acknowledge the assistance of Dr David Amaral of the Salk Institute in providing the services of his laboratory during the immunohistochemical portion of this study. Finally, we wish to thank Josephine Macenka of Caltech for expert technical assistance during many phases of this project. This work was supported by the Hixon Professorship of Psychobiology at Caltech, a Lucille B. Markey Charitable Trust Foundation grant, and a NRSA T32 GM07737 to C.T.M.
Funders:
Funding AgencyGrant Number
Hixson Professorship, CaltechUNSPECIFIED
Lucille P. Markey Charitable TrustUNSPECIFIED
NIH Predoctoral FellowshipT32 GM07737
Issue or Number:4
Record Number:CaltechAUTHORS:20200629-134857909
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20200629-134857909
Official Citation:C.T. McDonald, E.R. McGuinness, J.M. Allman, Laminar organization of acetylcholinesterase and cytochrome oxidase in the lateral geniculate nucleus of prosimians, Neuroscience, Volume 54, Issue 4, 1993, Pages 1091-1101, ISSN 0306-4522, https://doi.org/10.1016/0306-4522(93)90598-A.
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:104143
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:29 Jun 2020 21:10
Last Modified:29 Jun 2020 21:10

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