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Secondary single-cell transcriptomic analysis reveals common molecular signatures of cerebrovascular injury between traumatic brain injury and aging

Guo, Xinying and Zhang, Bangyan and Gomez-Pinilla, Fernando and Gao, Fan and Zhao, Zhen (2020) Secondary single-cell transcriptomic analysis reveals common molecular signatures of cerebrovascular injury between traumatic brain injury and aging. . (Unpublished) https://resolver.caltech.edu/CaltechAUTHORS:20200701-103607821

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Abstract

Cerebrovascular injury is a common pathological feature of a spectrum of neurological disorders including traumatic brain injury (TBI), stroke, Alzheimer's disease (AD), as well as aging. Vascular manifestations among these conditions are similar indeed, including the breakdown of the blood-brain barrier (BBB). However, whether there is a common molecular mechanism underlying the vascular changes among these conditions remains elusive. Here, we report secondary transcriptomic analysis on cerebrovascular cells based single-cell RNA-seq datasets of mouse models of mild TBI and aging, with a focus on endothelial cells and pericytes. We identify several molecular signatures commonly found between mTBI and aging vasculature, including Adamts1, Rpl23a, Tmem252, Car4, Serpine2, and Ndnf in endothelial cells, and Rps29 and Sepp1 in pericytes. These markers may represent the shared endophenotype of microvascular injury and be considered as cerebrovascular injury responsive genes. Additionally, pathway analysis on differentially expressed genes demonstrated alterations in common pathways between mTBI and aging, including vascular development and extracellular matrix pathways in endothelial cells. Hence, our analysis suggests that cerebrovascular injury triggered by different neurological conditions may share common molecular signatures, which may only be detected at the single-cell transcriptome level.


Item Type:Report or Paper (Discussion Paper)
Related URLs:
URLURL TypeDescription
https://doi.org/10.1101/2020.06.29.178855DOIDiscussion Paper
Additional Information:The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license. The authors have declared no competing interest.
Subject Keywords:Single cell RNA sequencing analysis, Traumatic brain injury, Alzheimer’s disease, microvascular injury, blood-brain barrier
Record Number:CaltechAUTHORS:20200701-103607821
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20200701-103607821
Official Citation:Secondary single-cell transcriptomic analysis reveals common molecular signatures of cerebrovascular injury between traumatic brain injury and aging Xinying Guo, Bangyan Zhang, Fernando Gomez-Pinilla, Fan Gao, Zhen Zhao bioRxiv 2020.06.29.178855; doi: https://doi.org/10.1101/2020.06.29.178855
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:104186
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:01 Jul 2020 20:10
Last Modified:01 Jul 2020 20:10

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