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Ambient PM Toxicity is Correlated with Expression Levels of Specific MicroRNAs

Chen, Haoxuan and Zhang, Xiangyu and Zhang, Ting and Li, Xinyue and Li, Jing and Yue, Yang and Wang, Minfei and Zheng, Yunhao and Fan, Hanqing and Wang, Jing and Yao, Maosheng (2020) Ambient PM Toxicity is Correlated with Expression Levels of Specific MicroRNAs. Environmental Science and Technology, 54 (16). pp. 10227-10236. ISSN 0013-936X. https://resolver.caltech.edu/CaltechAUTHORS:20200714-095127191

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Abstract

Uncertainties regarding optimized air pollution control remain as the underlying mechanisms of city-specific ambient particulate matter (PM)-induced health effects are unknown. Here, water-soluble extracts of PMs collected from four global cities via automobile air-conditioning filters were consecutively injected three times by an amount of 1, 2, and 2 mg into the blood circulation of Wistar rats after filtration by a 0.45 μm pore size membrane. Acute health effects, such as immune and inflammatory responses and hemorrhage in alveoli, were observed right after the PM extraction injection. Significant differences between cities in biomarker tumor necrosis factor-α (TNF-α) and monocyte chemoattractant protein-1 (MCP-1) levels were detected following the second and third PM injections. Rats’ inflammatory responses varied substantially with the injections of city-specific PMs. Repeated PM extract exposure rendered the rats more vulnerable to subsequent challenges, and downregulation of certain microRNAs was observed in rats. Among the studied miRNAs, miR-125b, and miR-21 were most sensitive to the PM exposure, exhibiting a negative dose–response-type relationship with a source-specific PM (oxidative potential) toxicity (r² = 0.63 and 0.57; p-values < 0.05). The results indicated that city-specific PMs could induce different health effects by selectively regulating different miRNAs, and that certain microRNAs, e.g., miR-125b and miR-21, may be externally mediated to neutralize PM-related health damages.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1021/acs.est.0c03876DOIArticle
ORCID:
AuthorORCID
Chen, Haoxuan0000-0001-8398-9093
Zheng, Yunhao0000-0002-5788-886X
Wang, Jing0000-0003-2078-137X
Yao, Maosheng0000-0002-1442-8054
Additional Information:© 2020 American Chemical Society. Received: June 14, 2020; Revised: July 13, 2020; Accepted: July 13, 2020; Published: July 14, 2020. This study was supported by the NSFC Distinguished Young Scholars Fund Awarded to M.Y. (21725701) and the Ministry of Science and Technology (grants 2016YFC0207102 and 2015CB553401). The authors declare no competing financial interest. All animal experiments in this study were approved by the Laboratory Animal Ethics Committee of Peking University (grant number LA2017204) and were performed in accordance with the Guidelines for Animal Experiments of Peking University.
Funders:
Funding AgencyGrant Number
National Natural Science Foundation of China21725701
Ministry of Science and Technology (Taipei)2016YFC0207102
Ministry of Science and Technology (Taipei)2015CB553401
Subject Keywords:Particulate Matter; Toxicity; MicroRNA; Inflammation; Biomarker; Catheter-embedded Rat Model
Issue or Number:16
Record Number:CaltechAUTHORS:20200714-095127191
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20200714-095127191
Official Citation:Ambient PM Toxicity Is Correlated with Expression Levels of Specific MicroRNAs. Haoxuan Chen, Xiangyu Zhang, Ting Zhang, Xinyue Li, Jing Li, Yang Yue, Minfei Wang, Yunhao Zheng, Hanqing Fan, Jing Wang, and Maosheng Yao. Environmental Science & Technology 2020 54 (16), 10227-10236; DOI: 10.1021/acs.est.0c03876
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:104375
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:14 Jul 2020 17:21
Last Modified:25 Aug 2020 19:26

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