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Characterizing the diverse cells that associate with the developing commissures of the zebrafish forebrain

Schnabl, J. and Litz, M. P. H. and Schneider, C. and PenkoffLidbeck, N. and Bashiruddin, S. and Schwartz, M. S. and Alligood, K. and Devoto, S. H. and Barresi, M. J. F. (2020) Characterizing the diverse cells that associate with the developing commissures of the zebrafish forebrain. Developmental Neurobiology . ISSN 1932-8451. (In Press) https://resolver.caltech.edu/CaltechAUTHORS:20200720-125317774

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Abstract

During embryonic development of bilaterally symmetrical organisms, neurons send axons across the midline at specific points to connect the two halves of the nervous system with a commissure. Little is known about the cells at the midline that facilitate this tightly regulated process. We exploit the conserved process of vertebrate embryonic development in the zebrafish model system to elucidate the identity of cells at the midline that may facilitate postoptic (POC) and anterior commissure (AC) development. We have discovered that three different gfap+ astroglial cell morphologies persist in contact with pathfinding axons throughout commissure formation. Similarly, olig2+ progenitor cells occupy delineated portions of the postoptic and anterior commissures where they act as multipotent, neural progenitors. Moreover, we conclude that both gfap+ and olig2+ progenitor cells give rise to neuronal populations in both the telencephalon and diencephalon; however, these varied cell populations showed significant developmental timing differences between the telencephalon and diencephalon. Lastly, we also showed that fli1a+ mesenchymal cells migrate along the presumptive commissure regions before and during midline axon crossing. Furthermore, following commissure maturation, specific blood vessels formed at the midline of the POC and immediately ventral and parallel to the AC. This comprehensive account of the cellular populations that correlate with the timing and position of commissural axon pathfinding has supported the conceptual modeling and identification of the early forebrain architecture that may be necessary for proper commissure development.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1002/dneu.22801DOIArticle
https://doi.org/10.1101/2020.07.16.205153DOIDiscussion Paper
https://drive.google.com/drive/folders/1Vr9Dacy_BmFrWd5AkAC-DWtlooVB4TyD?usp=sharingRelated ItemData/Code
Additional Information:© 2020 Wiley. Accepted manuscript online: 14 December 2020. We would like to thank Alicia Famiglietti, Cassie Kemmler, Narendra Pathak, Risha Sinha, Rachael Stein, Carla Valez, and Paula Zaman for all their supportive contributions during the course of this research. We would also like to thank the Smith College Center for Microscopy and its manager, Judith Wopereis, and the Smith Animal Care facility for their constant support and technical assistance throughout this work. Lastly, we are extremely appreciative of all constructive discourse provided by the entire Barresi lab from the first experiments to the final manuscript. This research was generously supported by the National Science Foundation [IOS-1656310] and by Smith College for both undergraduate and graduate student support. The authors declare no conflicts of interest.
Funders:
Funding AgencyGrant Number
NSFIOS-1656310
Smith CollegeUNSPECIFIED
Subject Keywords:commissure; forebrain; Gfap; glia; zebrafish
Record Number:CaltechAUTHORS:20200720-125317774
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20200720-125317774
Official Citation:Schnabl, J., Litz, M., Schneider, C., PenkoffLidbeck, N., Bashiruddin, S., Schwartz, M., Alligood, K., Devoto, S. and Barresi, M. (2020), Characterizing the diverse cells that associate with the developing commissures of the zebrafish forebrain. Developmental Neurobiology. Accepted Author Manuscript. https://doi.org/10.1002/dneu.22801
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:104454
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:20 Jul 2020 21:03
Last Modified:16 Dec 2020 19:44

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