CaltechAUTHORS
  A Caltech Library Service

Molecularly Engineered “Janus GroEL”: Application to Supramolecular Copolymerization with a Higher Level of Sequence Control

Kashiwagi, Daiki and Shen, Hao K. and Sim, Seunghyun and Sano, Koki and Ishida, Yasuhiro and Kimura, Ayumi and Niwa, Tatsuya and Taguchi, Hideki and Aida, Takuzo (2020) Molecularly Engineered “Janus GroEL”: Application to Supramolecular Copolymerization with a Higher Level of Sequence Control. Journal of the American Chemical Society, 142 (31). pp. 13310-13315. ISSN 0002-7863. https://resolver.caltech.edu/CaltechAUTHORS:20200721-100620809

[img] PDF - Accepted Version
See Usage Policy.

5Mb
[img] PDF (General methods, preparation and characterization of DNA-appended GroELs, additional TEM images, and computed fast Fourier transform analysis of a TEM image) - Supplemental Material
See Usage Policy.

19Mb

Use this Persistent URL to link to this item: https://resolver.caltech.edu/CaltechAUTHORS:20200721-100620809

Abstract

Herein we report the synthesis and isolation of a shape-persistent Janus protein nanoparticle derived from the biomolecular machine chaperonin GroEL (^AGroEL^B) and its application to DNA-mediated ternary supramolecular copolymerization. To synthesize ^AGroEL^B with two different DNA strands A and B at its opposite apical domains, we utilized the unique biological property of GroEL, i.e., Mg²⁺/ATP-mediated ring exchange between ^AGroEL^A and ^BGroEL^B with their hollow cylindrical double-decker architectures. This exchange event was reported more than 24 years ago but has never been utilized for molecular engineering of GroEL. We leveraged DNA nanotechnology to purely isolate Janus ^AGroEL^B and succeeded in its precision ternary supramolecular copolymerization with two DNA comonomers, A** and B*, that are partially complementary to A and B in ^AGroEL^B, respectively, and programmed to self-dimerize on the other side. Transmission electron microscopy allowed us to confirm the formation of the expected dual-periodic copolymer sequence −(^(B*/B)GroEL^(A/A**/A**/A)GroEL^(B/B*))– in the form of a laterally connected lamellar assembly rather than a single-chain copolymer.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1021/jacs.0c05937DOIArticle
ORCID:
AuthorORCID
Ishida, Yasuhiro0000-0002-5526-6100
Taguchi, Hideki0000-0002-6612-9339
Additional Information:© 2020 American Chemical Society. Received: June 1, 2020; Published: July 21, 2020. Author Contributions: D.K. and H.K.S. contributed equally. The authors declare no competing financial interest. This work was supported by a Grant-in-Aid for Scientific Research (S) (18H05260 to T.A.) and the Nanotechnology Platform Project of the Ministry of Education, Culture, Sports, Science and Technology of Japan (JPMXP09A18UT0027 and JPMXP09A20UT0042). We thank Mr. Hoshi for his assistance with transmission electron microscopy. H.K.S. thanks the Takayama International Education Foundation for a scholarship. S.S. thanks the Helen Hay Whitney Foundation for their support.
Funders:
Funding AgencyGrant Number
Japan Society for the Promotion of Science (JSPS)18H05260
Ministry of Education, Culture, Sports, Science and Technology (MEXT)JPMXP09A18UT0027
Ministry of Education, Culture, Sports, Science and Technology (MEXT)JPMXP09A20UT0042
Takayama International Education FoundationUNSPECIFIED
Helen Hay Whitney FoundationUNSPECIFIED
Issue or Number:31
Record Number:CaltechAUTHORS:20200721-100620809
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20200721-100620809
Official Citation:Molecularly Engineered “Janus GroEL”: Application to Supramolecular Copolymerization with a Higher Level of Sequence Control. Daiki Kashiwagi, Hao K. Shen, Seunghyun Sim, Koki Sano, Yasuhiro Ishida, Ayumi Kimura, Tatsuya Niwa, Hideki Taguchi, and Takuzo Aida. Journal of the American Chemical Society 2020 142 (31), 13310-13315; DOI: 10.1021/jacs.0c05937
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:104474
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:21 Jul 2020 17:28
Last Modified:06 Aug 2020 20:43

Repository Staff Only: item control page