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Translesion synthesis polymerases contribute to meiotic chromosome segregation and cohesin dynamics in Schizosaccharomyces pombe

Mastro, Tara L. and Tripathi, Vishnu P. and Forsburg, Susan L. (2020) Translesion synthesis polymerases contribute to meiotic chromosome segregation and cohesin dynamics in Schizosaccharomyces pombe. Journal of Cell Science, 133 (10). Art. No. jcs238709. ISSN 0021-9533. PMCID PMC7325440. https://resolver.caltech.edu/CaltechAUTHORS:20200723-122439156

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Abstract

Translesion synthesis polymerases (TLSPs) are non-essential error-prone enzymes that ensure cell survival by facilitating DNA replication in the presence of DNA damage. In addition to their role in bypassing lesions, TLSPs have been implicated in meiotic double-strand break repair in several systems. Here, we examine the joint contribution of four TLSPs to meiotic progression in the fission yeast Schizosaccharomyces pombe. We observed a dramatic loss of spore viability in fission yeast lacking all four TLSPs, which is accompanied by disruptions in chromosome segregation during meiosis I and II. Rec8 cohesin dynamics are altered in the absence of the TLSPs. These data suggest that the TLSPs contribute to multiple aspects of meiotic chromosome dynamics.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1242/jcs.238709DOIArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/pmc7325440/PubMed CentralArticle
ORCID:
AuthorORCID
Mastro, Tara L.0000-0003-1302-9753
Tripathi, Vishnu P.0000-0002-6137-8547
Forsburg, Susan L.0000-0002-4895-8598
Additional Information:© 2020 The author(s). Published by The Company of Biologists Ltd. Received 4 September 2019; Accepted 26 March 2020. Published online May 22, 2020. We thank members of the laboratory and Rachel Nieto for helpful comments. This work was supported by funding to S.L.F. from the National Institutes of Health (NIGMS R35 GM118109). Deposited in PMC for release after 12 months. Author contributions Conceptualization: T.L.M., S.L.F.; Methodology: T.L.M., S.L.F.; Validation: T.L.M., V.P.T.; Formal analysis: T.L.M.; Investigation: T.L.M., V.P.T.; Resources: S.L.F.; Writing - original draft: T.L.M.; Writing - review & editing: V.P.T., S.L.F.; Visualization: T.L.M.; Supervision: S.L.F.; Funding acquisition: S.L.F. The authors declare no competing or financial interests.
Funders:
Funding AgencyGrant Number
NIHR35 GM118109
Subject Keywords:DNA repair, Meiosis, Translesion synthesis, S. pombe, Rec8, DDK
Issue or Number:10
PubMed Central ID:PMC7325440
Record Number:CaltechAUTHORS:20200723-122439156
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20200723-122439156
Official Citation:Translesion synthesis polymerases contribute to meiotic chromosome segregation and cohesin dynamics in Schizosaccharomyces pombe Tara L. Mastro, Vishnu P. Tripathi, Susan L. Forsburg Journal of Cell Science 2020 133: jcs238709 doi: 10.1242/jcs.238709 Published 22 May 2020
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:104533
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:24 Jul 2020 14:25
Last Modified:24 Jul 2020 14:25

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