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Enabling out-of-clinic human immunity studies via single-cell profiling of capillary blood

Dobreva, Tatyana and Brown, David and Park, Jong Hwee and Thomson, Matt (2020) Enabling out-of-clinic human immunity studies via single-cell profiling of capillary blood. . (Unpublished) https://resolver.caltech.edu/CaltechAUTHORS:20200727-093309639

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Abstract

An individual's immune system is driven by both genetic and environmental factors that vary over time. To better understand the temporal and inter-individual variability of gene expression within distinct immune cell types, we developed a platform that leverages multiplexed single-cell sequencing and out-of-clinic capillary blood extraction to enable simplified, cost-effective profiling of the human immune system across people and time at single-cell resolution. Using the platform, we detect widespread differences in cell type-specific gene expression between subjects that are stable over multiple days.


Item Type:Report or Paper (Discussion Paper)
Related URLs:
URLURL TypeDescription
https://doi.org/10.1101/2020.07.25.210468DOIDiscussion Paper
https://data.caltech.edu/records/1407DOIData
https://github.com/thomsonlab/capblood-seqRelated ItemCode
http://capblood-seq.caltech.edu/#Related ItemData/Code
ORCID:
AuthorORCID
Dobreva, Tatyana0000-0002-2625-8873
Brown, David0000-0002-9757-1744
Additional Information:The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license. Posted July 26, 2020. The authors have declared no competing interest. Data Availability: Gene expression matrix and relevant metadata are available on https://data.caltech.edu/records/1407. Genome and FASTQ files are not being released to protect the identity of the subjects. Code Availability: Custom code made for diurnal and subject specific gene detection is available on https://github.com/thomsonlab/capblood-seq.
Record Number:CaltechAUTHORS:20200727-093309639
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20200727-093309639
Official Citation:Enabling out-of-clinic human immunity studies via single-cell profiling of capillary blood. Tatyana Dobreva, David Brown, Jong Hwee Park, Matt Thomson. bioRxiv 2020.07.25.210468; doi: https://doi.org/10.1101/2020.07.25.210468
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:104585
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:28 Jul 2020 16:17
Last Modified:28 Jul 2020 16:17

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