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Dorsal raphe dopamine neurons signal motivational salience dependent on internal and external states

Cho, Jounhong Ryan and Chen, Xinhong and Kahan, Anat and Robinson, J. Elliott and Wagenaar, Daniel A. and Gradinaru, Viviana (2020) Dorsal raphe dopamine neurons signal motivational salience dependent on internal and external states. . (Unpublished) https://resolver.caltech.edu/CaltechAUTHORS:20200728-094010838

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Abstract

The ability to recognize motivationally salient events and respond to them adaptively is critical for survival. Here we tested whether dopamine (DA) neurons in the dorsal raphe nucleus (DRN) contribute to this process. Population recordings of DRN^(DA) neurons during associative learning tasks showed that their activity dynamically tracks salience, developing excitation to both reward- and punishment-paired cues. The DRNDA response to reward-predicting cues was diminished after satiety, suggesting modulation by internal states. DRN^(DA) activity was also greater for unexpected outcomes than for expected outcomes. Two-photon imaging of DRN^(DA) neurons demonstrated that the majority of individual neurons developed activation to reward predicting cues but not to punishment-predicting cues, which was surprising and qualitatively distinct from the population results. Head-fixation during fear learning abolished the neural response to aversive cues, indicating modulation by behavioral context. Overall, these results suggest that DRN^(DA) neurons encode motivational salience, dependent on internal and external factors.


Item Type:Report or Paper (Discussion Paper)
Related URLs:
URLURL TypeDescription
https://doi.org/10.1101/2020.07.27.222729DOIDiscussion Paper
http://github.com/GradinaruLab/dLight1Related ItemData/Code
ORCID:
AuthorORCID
Cho, Jounhong Ryan0000-0001-9542-716X
Chen, Xinhong0000-0003-0408-0813
Wagenaar, Daniel A.0000-0002-6222-761X
Gradinaru, Viviana0000-0001-5868-348X
Additional Information:The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license. Posted July 27, 2020. We appreciate the entire Gradinaru lab for critical feedback. This work is supported by NIH Director’s New Innovator IDP20D091182-01, PECASE, NIH/NIA 1R01AG047664-01, NIH BRAIN 1U01NS090577, Heritage Medical Research Institute, Chen Institute (to V.G.), Beckman Institute (to V.G. and D.A.W.), TCCI Chen Graduate Fellowship (to X.C.), Colvin divisional fellowship at Caltech (to A.K.) and Children’s Tumor Foundation Young Investigator Award 2016-01-00 (to J.E.R). Data and materials availability: Codes for fiber photometry signal extraction and processing are available at http://github.com/GradinaruLab/dLight1. Source data with statistical results is available as a separate excel file. We have no competing interest to declare.
Group:Heritage Medical Research Institute, Tianqiao and Chrissy Chen Institute for Neuroscience
Funders:
Funding AgencyGrant Number
NIHIDP20D091182-01
NSFUNSPECIFIED
NIH1R01AG047664-01
NIH1U01NS090577
Heritage Medical Research InstituteUNSPECIFIED
Tianqiao and Chrissy Chen Institute for NeuroscienceUNSPECIFIED
Caltech Beckman InstituteUNSPECIFIED
Caltech Division of Biology and Biological EngineeringUNSPECIFIED
Children’s Tumor Foundation2016-01-00
Record Number:CaltechAUTHORS:20200728-094010838
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20200728-094010838
Official Citation:Dorsal raphe dopamine neurons signal motivational salience dependent on internal and external states. Jounhong Ryan Cho, Xinhong Chen, Anat Kahan, Elliott Robinson, Daniel A Wagenaar, Viviana Gradinaru. bioRxiv 2020.07.27.222729; doi: https://doi.org/10.1101/2020.07.27.222729
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:104602
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:28 Jul 2020 19:56
Last Modified:28 Jul 2020 19:56

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