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Study the property of double-ended fluoroalkyl poly(ethylene glycol) hydrogel as a depot for hydrophobic drug delivery using electron paramagnetic resonance technique and cell proliferation assay

Liu, Xiangli and Mao, Yougang and Mathias, Errol V. and Ma, Collin and Franco, Osmundo and Ba, Yong and Kornfield, Julia A. and Wang, Tieli and Xue, Lijun and Zhou, Bing-Sen and Yen, Yun (2008) Study the property of double-ended fluoroalkyl poly(ethylene glycol) hydrogel as a depot for hydrophobic drug delivery using electron paramagnetic resonance technique and cell proliferation assay. Journal of Sol-Gel Science and Technology, 45 (3). pp. 269-278. ISSN 0928-0707. https://resolver.caltech.edu/CaltechAUTHORS:20200728-174942242

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Abstract

Hydrogel formed by fluoroalkyl double-ended polyethylene glycol (R_f-PEG) micelles was studied to assess its properties to encapsulate a hydrophobic electron spin labeled drug, Chlorambucil–Tempol adduct (CT), and to control and sustain the drug release. The drug loaded hydrogel samples were characterized with variable-temperature dependent EPR experiment, and EPR theoretical lineshape analysis. It was found that CT molecules reside in the hydrophobic R_f-cores/IPDU shells of the R_f-PEG micelles and the maximum molecular-level loading capacity was estimated to be 18.8 mg per gram of the R_f-PEG. It has been known that R_f-PEG hydrogel with certain molecular masses for the fluoroalkyl group and the PEG chain shows properties of sol/gel phase coexistence and surface erosion, which represent the favorable condition for a pharmaceutical depot to control the kinetics of drug release. To evaluate the R_f-PEG’s biocompatibility and kinetics of the drug release, a cell proliferation assay was carried out on human oropharyngeal carcinoma (KB) cells. The results show that R_f-PEG is biocompatible and able to release CT to the cell media with a constant equilibrium concentration independent of the amount of CT loaded hydrogel.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1007/s10971-007-1659-yDOIArticle
https://rdcu.be/b6a1GPublisherFree ReadCube access
ORCID:
AuthorORCID
Kornfield, Julia A.0000-0001-6746-8634
Additional Information:© Springer Science+Business Media, LLC 2007. Received: 31 August 2007; Accepted: 12 November 2007; Published online: 13 December 2007. This project is supported by the NSF Grant 0351848. Thanks to DOD for funding the EPR spectrometer under the grant d20030904hsi.
Funders:
Funding AgencyGrant Number
NSF0351841
Department of Defensed2003090hsi
Subject Keywords:Polymeric drug depot, Rf-PEG hydrogel, Controlled release, Tempol, Chlorambucil, Proliferation assay, Electron paramagnetic resonance
Issue or Number:3
Record Number:CaltechAUTHORS:20200728-174942242
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20200728-174942242
Official Citation:Liu, X., Mao, Y., Mathias, E.V. et al. Study the property of double-ended fluoroalkyl poly(ethylene glycol) hydrogel as a depot for hydrophobic drug delivery using electron paramagnetic resonance technique and cell proliferation assay. J Sol-Gel Sci Technol 45, 269–278 (2008). https://doi.org/10.1007/s10971-007-1659-y
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:104626
Collection:CaltechAUTHORS
Deposited By: Rebecca Minjarez
Deposited On:10 Aug 2020 20:54
Last Modified:10 Aug 2020 20:54

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