Liu, Xiangli and Mao, Yougang and Mathias, Errol V. and Ma, Collin and Franco, Osmundo and Ba, Yong and Kornfield, Julia A. and Wang, Tieli and Xue, Lijun and Zhou, Bing-Sen and Yen, Yun (2008) Study the property of double-ended fluoroalkyl poly(ethylene glycol) hydrogel as a depot for hydrophobic drug delivery using electron paramagnetic resonance technique and cell proliferation assay. Journal of Sol-Gel Science and Technology, 45 (3). pp. 269-278. ISSN 0928-0707. https://resolver.caltech.edu/CaltechAUTHORS:20200728-174942242
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Abstract
Hydrogel formed by fluoroalkyl double-ended polyethylene glycol (R_f-PEG) micelles was studied to assess its properties to encapsulate a hydrophobic electron spin labeled drug, Chlorambucil–Tempol adduct (CT), and to control and sustain the drug release. The drug loaded hydrogel samples were characterized with variable-temperature dependent EPR experiment, and EPR theoretical lineshape analysis. It was found that CT molecules reside in the hydrophobic R_f-cores/IPDU shells of the R_f-PEG micelles and the maximum molecular-level loading capacity was estimated to be 18.8 mg per gram of the R_f-PEG. It has been known that R_f-PEG hydrogel with certain molecular masses for the fluoroalkyl group and the PEG chain shows properties of sol/gel phase coexistence and surface erosion, which represent the favorable condition for a pharmaceutical depot to control the kinetics of drug release. To evaluate the R_f-PEG’s biocompatibility and kinetics of the drug release, a cell proliferation assay was carried out on human oropharyngeal carcinoma (KB) cells. The results show that R_f-PEG is biocompatible and able to release CT to the cell media with a constant equilibrium concentration independent of the amount of CT loaded hydrogel.
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| Additional Information: | © Springer Science+Business Media, LLC 2007. Received: 31 August 2007; Accepted: 12 November 2007; Published online: 13 December 2007. This project is supported by the NSF Grant 0351848. Thanks to DOD for funding the EPR spectrometer under the grant d20030904hsi. | |||||||||
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| Subject Keywords: | Polymeric drug depot, Rf-PEG hydrogel, Controlled release, Tempol, Chlorambucil, Proliferation assay, Electron paramagnetic resonance | |||||||||
| Issue or Number: | 3 | |||||||||
| Record Number: | CaltechAUTHORS:20200728-174942242 | |||||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20200728-174942242 | |||||||||
| Official Citation: | Liu, X., Mao, Y., Mathias, E.V. et al. Study the property of double-ended fluoroalkyl poly(ethylene glycol) hydrogel as a depot for hydrophobic drug delivery using electron paramagnetic resonance technique and cell proliferation assay. J Sol-Gel Sci Technol 45, 269–278 (2008). https://doi.org/10.1007/s10971-007-1659-y | |||||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | |||||||||
| ID Code: | 104626 | |||||||||
| Collection: | CaltechAUTHORS | |||||||||
| Deposited By: | Rebecca Minjarez | |||||||||
| Deposited On: | 10 Aug 2020 20:54 | |||||||||
| Last Modified: | 10 Aug 2020 20:54 |
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