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Fatty acid metabolism in an oribatid mite: de novo biosynthesis and the effect of starvation

Brückner, Adrian and Heethoff, Michael (2020) Fatty acid metabolism in an oribatid mite: de novo biosynthesis and the effect of starvation. Experimental and Applied Acarology, 81 (4). pp. 483-494. ISSN 0168-8162. doi:10.1007/s10493-020-00529-8.

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The fatty acid (FA) composition of lipids in animals is influenced by factors such as species, life stage, availability and type of food, as well as the ability to synthesize certain FAs de novo. We investigated the effect of starvation on the neutral lipid (NLFA) and phospholipid (PLFA) fatty acid patterns of the oribatid mite Archegozetes longisetosus Aoki. Furthermore, we performed stable-isotope labeled precursors feeding experiments under axenic conditions to delineate de novo FA synthesis by profiling ¹³C and deuterium incorporation via single-ion monitoring. Starvation of mites resulted in a decline in the total amount of NLFAs and significantly changed the fatty acid patterns, indicating that NLFAs were metabolized selectively. Biochemical tracer experiments confirmed that oribatid mites, like other animals, can produce stearic (18:0) and oleic acid (18:1ω9) de novo. Mass spectrometric data also revealed that they appear to synthesize linoleic acid [18:2ω6,9 = (9Z,12Z)-octadeca-9,12-dienoic acid]—an ability restricted only to a few arthropod taxa, including astigmatid mites. The physiological and biosynthesis processes revealed here are crucial to understand the potential biomarker function of fatty acids—especially 18:2ω6,9—in oribatid mites and their applicability in soil animal food web studies.

Item Type:Article
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URLURL TypeDescription
Brückner, Adrian0000-0002-9184-8562
Heethoff, Michael0000-0003-3453-4871
Additional Information:© 2020 Springer Nature Switzerland AG. Received 20 May 2020; Accepted 27 July 2020; Published 03 August 2020. Adrian Brückner is a Simons Fellow of the Life Sciences Research Foundation. This study was supported by the German Research Foundation (DFG; HE 4593/5-1). AB and MH declare that they have no conflict of interest. Ethical approval: There are no legal restrictions on working with mites.
Funding AgencyGrant Number
Simons FoundationUNSPECIFIED
Deutsche Forschungsgemeinschaft (DFG)HE 4593/5-1
Subject Keywords:Neutral lipid fatty acids; Starvation; Stable-isotope labeling; Soil animal; Biochemistry
Issue or Number:4
Record Number:CaltechAUTHORS:20200803-153953850
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Official Citation:Brückner, A., Heethoff, M. Fatty acid metabolism in an oribatid mite: de novo biosynthesis and the effect of starvation. Exp Appl Acarol 81, 483–494 (2020).
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:104713
Deposited By: Tony Diaz
Deposited On:03 Aug 2020 22:57
Last Modified:05 Jan 2022 23:06

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