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DNA sequence coding for an antifreeze protein precursor from winter flounder

Davies, Peter L. and Roach, Arthur H. and Hew, Choy-L. (1982) DNA sequence coding for an antifreeze protein precursor from winter flounder. Proceedings of the National Academy of Sciences of the United States of America, 79 (2). pp. 335-339. ISSN 0027-8424.

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A cDNA made to antifreeze protein mRNA of the winter flounder was cloned in the plasmid pBR322 and its sequence was determined by the method of Maxam and Gilbert. Its sequence codes for a precursor protein that is 82 amino acids in length. This precursor has both a signal polypeptide and a prosequence before the mature protein of 38 amino acid residues. The mature protein matches in composition one of the alanine-rich serum antifreeze proteins that was purified by ion-exchange and reverse-phase chromatography. The composition of the pro-sequence is similar to that of the native protein except that it contains five prolines. The mature protein, but not the pro-sequence, contains three of the 11-residue repeats previously observed [Lin, Y. & Gross, J. K. (1981) Proc. Natl. Acad. Sci. USA 78, 2825-2829] in two other antifreeze protein components.

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Additional Information:© 1982 by the National Academy of Sciences. Communicated by Norman H. Horowitz, September 28, 1981. We thank Donna Stapleton and Christine Hough-McElhanney for technical assistance and Dr. Geoff Flynn for his advice and his help in performing the automated Edman degradation. We are also grateful to Dr. Joseph Beard and the National Cancer Institute for the gift of reverse transcriptase. This work was supported by grants to P.L.D. and C.-L.H. from the Medical Research Council of Canada and by the award of a Medical Research Council Scholarship to P.L.D. This is contribution 435 from the Marine Sciences Research Laboratory, Memorial University of Newfoundland. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
Subject Keywords:preproprotein; cDNA; recombinant DNA
Issue or Number:2
Record Number:CaltechAUTHORS:DAVpnas82
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:10474
Deposited By: Tony Diaz
Deposited On:09 May 2008
Last Modified:03 Oct 2019 00:09

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