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The SCF ubiquitin ligase protein Slimb regulates centrosome duplication in Drosophila

Wojcik, Edward J. and Glover, David M. and Hays, Thomas S. (2000) The SCF ubiquitin ligase protein Slimb regulates centrosome duplication in Drosophila. Current Biology, 10 (18). pp. 1131-1134. ISSN 0960-9822. doi:10.1016/s0960-9822(00)00703-x.

[img] PDF (Additional methodological detail and two figures showing that off-axis centrosomes in the slimbcrd mutant contain ASP, and that polyploid slimbcrd neuroblasts have bipolar spindles and excess centrosomes) - Supplemental Material
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The duplication of the centrosome is a key event in the cell-division cycle. Although defects in centrosome duplication are thought to contribute to genomic instability [1], [2], [3] and are a hallmark of certain transformed cells and human cancer [4], [5], [6], the mechanism responsible for centrosome duplication is not understood. Recent experiments have established that centrosome duplication requires the activity of cyclin-dependent kinase 2 (Cdk2) and cyclins E and A [7], [8], [9]. The stability of cyclin E is regulated by the ubiquitin ligase SCF, which is a protein complex composed of Skp1, Cdc53 (Cullin) and F-box proteins [10], [11], [12]. The Skp1 and Cullin components have been detected on mammalian centrosomes, and shown to be essential for centrosome duplication and separation in Xenopus[13]. Here, we report that Slimb, an F-box protein that targets proteins to the SCFcomplex [14], [15], plays a role in limiting centrosome replication. We found that, in the fruit fly Drosophila, the hypomorphic mutation slimbcrd causes the appearance of additional centrosomes and mitotic defects in mutant larval neuroblasts.

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Glover, David M.0000-0003-0956-0103
Additional Information:© 2000 Elsevier. Under an Elsevier user license. Received 10 March 2000, Revised 20 July 2000, Accepted 1 August 2000, Available online 13 September 2000. We thank Mark Sanders and David Gartner at the Imaging Center for assistance with the confocal microscope and digital image processing, and Will Whitfield (University of Dundee) for anti-CP190 antibody. This work was supported by grants from NIH (GM44757 to T.S.H. and GM19123 to E.J.W.) and the Cancer Research Campaign (D.M.G.).
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Cancer Research CampaignUNSPECIFIED
Issue or Number:18
Record Number:CaltechAUTHORS:20200806-163116379
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Official Citation:Edward J. Wojcik, David M. Glover, Thomas S. Hays, The SCF ubiquitin ligase protein Slimb regulates centrosome duplication in Drosophila, Current Biology, Volume 10, Issue 18, 2000, Pages 1131-1134, ISSN 0960-9822,
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:104795
Deposited By: George Porter
Deposited On:10 Aug 2020 17:36
Last Modified:16 Nov 2021 18:35

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