Bhat, Manzoor A. and Philp, Alastair Valentine and Glover, David M. and Bellen, Hugo J. (1996) Chromatid Segregation at Anaphase Requires the barren Product, a Novel Chromosome-Associated Protein That Interacts with Topoisomerase II. Cell, 87 (6). pp. 1103-1114. ISSN 0092-8674. doi:10.1016/s0092-8674(00)81804-8. https://resolver.caltech.edu/CaltechAUTHORS:20200806-163116487
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Abstract
We have isolated a Drosophila gene, barren (barr), required for sister-chromatid segregation in mitosis. barr encodes a novel protein that is present in proliferating cells and has homologs in yeast and human. Mitotic defects in barr embryos become apparent during cycle 16, resulting in a loss of PNS and CNS neurons. Centromeres move apart at the metaphase–anaphase transition and Cyclin B is degraded, but sister chromatids remain connected, resulting in chromatin bridging. This phenotype is similar to that described in TOP2 mutants in yeast. Barren protein localizes to chromatin throughout mitosis. Colocalization and biochemical experiments indicate that Barren associates with Topoisomerase II throughout mitosis and alters the activity of Topoisomerase II. We propose that this association is required for proper chromosomal segregation by facilitating the decatenation of chromatids at anaphase.
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| Additional Information: | © 1996 by Cell Press. Under an Elsevier user license. Received 10 June 1996, Revised 15 October 1996. We thank Doug Ruden, Isabella Molina, Minx Fuller, Pedro Ripoll, Hiro Ohkura, and Luke Alphey for fly strains and sequences and unpublished data. We thank the Indiana Stock Center and Trudi McKay for fly strains; Paul Fisher and David Agard for anti-TOPO II antibodies; Tao-Shih Hsieh for topo II cDNA, TOPO II protein, and suggestions; Jordan Raff forGAGAantibody; and Victoria Lundblad, Shelly Sazer, Mitzi Kuroda, Mark Wu, Sergei Prokopenko, and Emma Warbrick for comments on the manuscript. Special thanks to Victoria Lundblad for advice on TOPO II assays and to one of the reviewers for suggesting experiments described in Figures 6I and 6J. D. M. G. and A. V. P. acknowledge Core Grant support from the Cancer Research Campaign and Project Grant support from the Medical Research Council of Great Britain. M. A. B. is a research associate of the Howard Hughes Medical Institute. H. J. B. is an Associate Investigator from the Howard Hughes Medical Institute. | ||||||||
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| Issue or Number: | 6 | ||||||||
| DOI: | 10.1016/s0092-8674(00)81804-8 | ||||||||
| Record Number: | CaltechAUTHORS:20200806-163116487 | ||||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20200806-163116487 | ||||||||
| Official Citation: | Manzoor A Bhat, Alastair Valentine Philp, David M Glover, Hugo J Bellen, Chromatid Segregation at Anaphase Requires the barren Product, a Novel Chromosome-Associated Protein That Interacts with Topoisomerase II, Cell, Volume 87, Issue 6, 1996, Pages 1103-1114, ISSN 0092-8674, https://doi.org/10.1016/S0092-8674(00)81804-8. | ||||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | ||||||||
| ID Code: | 104796 | ||||||||
| Collection: | CaltechAUTHORS | ||||||||
| Deposited By: | George Porter | ||||||||
| Deposited On: | 10 Aug 2020 17:39 | ||||||||
| Last Modified: | 16 Nov 2021 18:35 |
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