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Microfabricated Gaps Reveal the Effect of Geometrical Control in Wound Healing

Bao, Min and Xie, Jing and Piruska, Aigars and Hu, Xinyu and Huck, Wilhelm T. S. (2020) Microfabricated Gaps Reveal the Effect of Geometrical Control in Wound Healing. Advanced Healthcare Materials . ISSN 2192-2640. (In Press) https://resolver.caltech.edu/CaltechAUTHORS:20200807-090347984

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Abstract

The geometry (size and shape) of gaps is a key determinant in controlling gap closure during wound healing. However, conventional methods for creating gaps result in un‐defined geometries and poorly characterized conditions (cell death factors and cell debris), which can influence the gap closure process. To overcome these limitations, a novel method to create well‐defined geometrical gaps is developed. First, smooth muscle cells (SMCs) are seeded in variously shaped micro‐containers made out of hyaluronic acid hydrogels. Cell proliferation and cell tension induce fibrous collagen production by SMCs predominantly around the edges of the micro‐containers. Upon removal of SMCs, the selectively deposited collagen results in micro‐containers with cell‐adhesive regions along the edges and walls. Fibroblasts are seeded in these micro‐containers, and upon attaching and spreading, they naturally form gaps with different geometries. The rapid proliferation of fibroblasts from the edge results in filling and closure of the gaps. It is demonstrated that gap closure rate as well as closure mechanism is strongly influenced by geometrical features, which points to an important role for cellular tension and cell proliferation in gap closure.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1002/adhm.202000630DOIArticle
ORCID:
AuthorORCID
Bao, Min0000-0003-0992-9388
Huck, Wilhelm T. S.0000-0003-4222-5411
Additional Information:© 2020 The Authors. Published by Wiley‐VCH GmbH. This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. Manuscript received: 17 April 2020. Manuscript revised: 26 June 2020. Version of Record online: 06 August 2020. M.B. and J.X. contributed equally to this work. The authors are grateful to the Department of General Instruments of the Radboud University for providing confocal and light microscopy services. The authors acknowledge financial support from the China Scholarship Council (J.X. and X.H.). The authors declare no conflict of interest.
Funders:
Funding AgencyGrant Number
China Scholarship CouncilUNSPECIFIED
Subject Keywords:hydrogels; wound healing; cell spreading; microniches
Record Number:CaltechAUTHORS:20200807-090347984
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20200807-090347984
Official Citation:Bao, M., Xie, J., Piruska, A., Hu, X., Huck, W. T. S., Microfabricated Gaps Reveal the Effect of Geometrical Control in Wound Healing. Adv. Healthcare Mater. 2020, 2000630. https://doi.org/10.1002/adhm.202000630
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:104812
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:10 Aug 2020 15:53
Last Modified:10 Aug 2020 15:53

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