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The structure and expression of the preproenkephalin gene

Legon, Steve and Glover, David M. and Hughes, John and Lowry, Philip J. and Rigby, Peter W. J. and Watson, Christine J. (1982) The structure and expression of the preproenkephalin gene. Nucleic Acids Research, 10 (24). pp. 7905-7918. ISSN 0305-1048. PMCID PMC327058. https://resolver.caltech.edu/CaltechAUTHORS:20200807-170350737

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Abstract

Enkephalins are pentapeptides with opioid activity which are found in a wide variety of tissues. Studies of enkephalin-containing peptides from the adrenal gland have established that the mature pentapeptides are derived by proteolytic processing of a precursor protein. We have shown that human adrenal medullary tumours contain mRNA which can be translated in vitro to yield a single major enkephalin precursor. The sequence of cloned cDNA shows that the preproenkephal in mRNA encodes four copies of met-enkephalin, two copies of the met-enkephalin extended sequences and one copy of leuenkephalin; each copy is flanked by paired basic amino acids which are presumably recognised by the processing protease. We have used the cloned human cDNA as a hybridization probe to detect the corresponding mRNAs in rat adrenal grand and, in smaller amounts, in rat brain. We have been unable to detect in brain any other cross-hybridising mRNAs which might encode other putative precursor proteins.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1093/nar/10.24.7905DOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC327058/PubMed CentralArticle
ORCID:
AuthorORCID
Glover, David M.0000-0003-0956-0103
Additional Information:© 1982 IRL Press. Creative Commons Attribution Non-Commercial licence (CC BY-NC). Received 16 November 1982; Accepted 30 November 1982. We are grateful to Doug Hanahan (Harvard University) for making pXf3 and E. coli DH1 available to us, to Mike Eaton (Celltech Ltd) for very generously synthesising the oligonucleotide probes and to Profs. G.M. Besser and L.H. Rees (St. Bartholomew's Hospital) for providing us with tumour samples. D.M.G. and P.W.J.R. hold Career Development Awards from the Cancer Research Campaign. This research was performed under a contract from the National Research Development Corporation.
Funders:
Funding AgencyGrant Number
Cancer Research CampaignUNSPECIFIED
National Research Development CorporationUNSPECIFIED
Issue or Number:24
PubMed Central ID:PMC327058
Record Number:CaltechAUTHORS:20200807-170350737
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20200807-170350737
Official Citation:Steve Legon, David M. Glover, John Hughes, Philip J. Lowry, Peter W. J. Rigby, Christine J. Watson, The structure and expression of the preproenkephalin gene, Nucleic Acids Research, Volume 10, Issue 24, 20 December 1982, Pages 7905–7918, https://doi.org/10.1093/nar/10.24.7905
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:104840
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:11 Aug 2020 17:30
Last Modified:11 Aug 2020 17:30

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