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Site specific insertion of a type I rDNA dement into a unique sequence in the Drosophila melanogaster genome

Browne, Michael J. and Read, Christopher A. and Roiha, Heli and Glover, David M. (1984) Site specific insertion of a type I rDNA dement into a unique sequence in the Drosophila melanogaster genome. Nucleic Acids Research, 12 (23). pp. 9111-9122. ISSN 0305-1048. PMCID PMC320441. doi:10.1093/nar/12.23.9111.

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We describe a cloned segment of unique DNA from the Oregon R strain of Drosophila melanogaster that contains a short type I insertion of the kind principally found within rDNA. The predominant type I rDNA insertion is 5kb in length, but there are also a co-terminal sub-set of shorter type I elements that share a common right hand junction with the rDNA. The insertion that we now describe is another member of this sub-set. The right hand junction of the type I sequence with the unique DNA is identical to the right hand junction of the type I sequences with rDNA. There is no significant feature within the insertion sequence that could have determined the position of the left junction with the sequence into which it is inserted. Like the corresponding short type I insertions in rDNA, the insertion into the unique DNA is flanked on both sides by a duplicated sequence, which in this case is 10 base pairs long. The cloning of a sequence corresponding to the uninterrupted unique location was facilitated by the observation that the Karsnas strain of D. melanoqaster contains only uninterrupted sequences of this kind. The duplicated sequence at the target site for the insertion is only present as a single copy in the uninterrupted DNA. The sequence of the target site for the insertion (ACTGTTCT) in the unique segment shows a striking homology to the target in rDNA (ACTGTCCC).

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Glover, David M.0000-0003-0956-0103
Additional Information:© 1984 IRL Press Limited. Creative Commons Attribution Non-Commercial licence (CC BY-NC). Received 11 October 1984; Revised and Accepted 8 November 1984. This work was supported by grants from the Medical Research Council and the Cancer Research Campaign (C.R.C). D.M.G. holds a Career Development Award from the C.R.C. M.J.B. was a visiting worker from Beechams Pharmaceuticals during 1981.
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Medical Research Council (UK)UNSPECIFIED
Cancer Research CampaignUNSPECIFIED
Issue or Number:23
PubMed Central ID:PMC320441
Record Number:CaltechAUTHORS:20200807-170350920
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Official Citation:Michael J. Browne, Christopher A. Read, Heli Roiha, David M. Glover, Site specific insertion of a type I rDNA dement into a unique sequence in the Drosophila melanogaster genome , Nucleic Acids Research, Volume 12, Issue 23, 11 December 1984, Pages 9111–9122,
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:104842
Deposited By: George Porter
Deposited On:11 Aug 2020 17:37
Last Modified:16 Nov 2021 18:36

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