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Analysis of the Drosophila rDNA promoter by transient expression

Hayward, David C. and Glover, David M. (1988) Analysis of the Drosophila rDNA promoter by transient expression. Nucleic Acids Research, 16 (10). pp. 4253-4268. ISSN 0305-1048. PMCID PMC336628. doi:10.1093/nar/16.10.4253. https://resolver.caltech.edu/CaltechAUTHORS:20200807-170351129

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Abstract

We have examined the expression of the bacterial gene chloramphenicol acetyl transferase (CAT) under the control of the Drosophila rDNA promoter following transfection into Drosophila tissue culture cells. Constructs having an entire NTS, corresponding to approximately 3640 base pairs of upstream rDNA sequence, or constructs with 306 base pairs of upstream sequence respectively, are transcribed at 5 fold or 2 fold higher levels than a construct with 43 base pairs of upstream DNA. In co-transfection experiments, the construct with the entire NTS competes for transcription 20 fold more effectively than the construct with 306 base pairs of upstream sequence. Constructs having either 72 base pairs or 60 base pairs of upstream rDNA sequences, on the other hand, are transcribed very much less efficiently than constructs with either 306 bp or with only 43 bp of upstream DNA. These sequences, which reduce levels of rDNA transcription in the absence of additional upstream DNA, lie in a region in which the rDNA promoter differs from its duplications within the NTS.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1093/nar/16.10.4253DOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC336628/PubMed CentralArticle
ORCID:
AuthorORCID
Glover, David M.0000-0003-0956-0103
Additional Information:© 1988 IRL Press Limited. Creative Commons Attribution Non-Commercial licence (CC BY-NC). Received March 3, 1988; Revised and Accepted April 22, 1988. We would like to thank Peter Rae and Bruce Kohorn for providing us with plasmids. We are grateful to the Cancer Research Campaign for supporting all aspects of this work including a Career Development Award to DMG and a Research Studentship to DCH.
Funders:
Funding AgencyGrant Number
Cancer Research CampaignUNSPECIFIED
Issue or Number:10
PubMed Central ID:PMC336628
DOI:10.1093/nar/16.10.4253
Record Number:CaltechAUTHORS:20200807-170351129
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20200807-170351129
Official Citation:David C. Hayward, David M. Glover, Analysis of the Drosophil a rDNA promoter by transient expression , Nucleic Acids Research, Volume 16, Issue 10, 25 May 1988, Pages 4253–4268, https://doi.org/10.1093/nar/16.10.4253
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:104844
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:11 Aug 2020 19:17
Last Modified:16 Nov 2021 18:36

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