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Molecular cloning and developmental expression of the catalytic and 65-kDa regulatory subunits of protein phosphatase 2A in Drosophila

Mayer-Jaekel, Regina E. and Baumgartner, Stefan and Bilbe, Graeme and Ohkura, Hiroyuki and Glover, David M. and Hemmings, Brian A. (1992) Molecular cloning and developmental expression of the catalytic and 65-kDa regulatory subunits of protein phosphatase 2A in Drosophila. Molecular Biology of the Cell, 3 (3). pp. 287-298. ISSN 1059-1524. PMCID PMC275530. doi:10.1091/mbc.3.3.287.

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cDNA clones encoding the catalytic subunit and the 65-kDa regulatory subunit of protein phosphatase 2A (PR65) from Drosophila melanogaster have been isolated by homology screening with the corresponding human cDNAs. The Drosophila clones were used to analyze the spatial and temporal expression of the transcripts encoding these two proteins. The Drosophila PR65 cDNA clones contained an open reading frame of 1773 nucleotides encoding a protein of 65.5 kDa. The predicted amino acid sequence showed 75 and 71% identity to the human PR65 alpha and beta isoforms, respectively. As previously reported for the mammalian PR65 isoforms, Drosophila PR65 is composed of 15 imperfect repeating units of approximately 39 amino acids. The residues contributing to this repeat structure show also the highest sequence conservation between species, indicating a functional importance for these repeats. The gene encoding Drosophila PR65 was located at 29B1,2 on the second chromosome. A major transcript of 2.8 kilobase (kb) encoding the PR65 subunit and two transcripts of 1.6 and 2.5 kb encoding the catalytic subunit could be detected throughout Drosophila development. All of these mRNAs were most abundant during early embryogenesis and were expressed at lower levels in larvae and adult flies. In situ hybridization of different developmental stages showed a colocalization of the PR65 and catalytic subunit transcripts. The mRNA expression is high in the nurse cells and oocytes, consistent with a high equally distributed expression in early embryos. In later embryonal development, the expression remains high in the nervous system and the gonads but the overall transcript levels decrease. In third instar larvae, high levels of mRNA could be observed in brain, imaginal discs, and in salivary glands. These results indicate that protein phosphatase 2A transcript levels change during development in a tissue and in a time-specific manner.

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Glover, David M.0000-0003-0956-0103
Additional Information:© 1992 by The American Society for Cell Biology. Submitted November 1, 1991; Accepted December 24, 1991. We are grateful to Drs. Tom Komberg and Bemd Hovemann for providing the cDNA libraries. We thank Francis Maurer and Peter Cron for cheerful technical assistance, Franz Fischer for the oligonucleotide synthesis, and Drs. Bertrand Favre, Jan Hofsteenge, and Annette Brice for helpful comments on the manuscript. Dr. Pam Jones is thanked for her help and indispensable discussions during the course of this work. D.M.G. and H.O. are grateful to the Cancer Research Campaign and the Human Frontiers Science Programme for support.
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Cancer Research CampaignUNSPECIFIED
Human Frontier Science ProgramUNSPECIFIED
Issue or Number:3
PubMed Central ID:PMC275530
Record Number:CaltechAUTHORS:20200807-170351589
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Official Citation:Molecular cloning and developmental expression of the catalytic and 65-kDa regulatory subunits of protein phosphatase 2A in Drosophila. R E Mayer-Jaekel, S Baumgartner, G Bilbe, H Ohkura, D M Glover, and B A Hemmings Molecular Biology of the Cell 1992 3:3, 287-298
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:104848
Deposited By: George Porter
Deposited On:11 Aug 2020 20:47
Last Modified:16 Nov 2021 18:36

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