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Drosophila Aurora A kinase is required to localize D-TACC to centrosomes and to regulate astral microtubules

Giet, Régis and McLean, Doris and Descamps, Simon and Lee, Michael J. and Raff, Jordan W. and Prigent, Claude and Glover, David M. (2002) Drosophila Aurora A kinase is required to localize D-TACC to centrosomes and to regulate astral microtubules. Journal of Cell Biology, 156 (3). pp. 437-451. ISSN 1540-8140. PMCID PMC2173350. doi:10.1083/jcb.200108135. https://resolver.caltech.edu/CaltechAUTHORS:20200807-170352216

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Abstract

Disruption of the function of the A-type Aurora kinase of Drosophila by mutation or RNAi leads to a reduction in the length of astral microtubules in syncytial embryos, larval neuroblasts, and cultured S2 cells. In neuroblasts, it can also lead to loss of an organized centrosome and its associated aster from one of the spindle poles, whereas the centrosome at the other pole has multiple centrioles. When centrosomes are present at the poles of aurA mutants or aurA RNAi spindles, they retain many antigens but are missing the Drosophila counterpart of mammalian transforming acidic coiled coil (TACC) proteins, D-TACC. We show that a subpopulation of the total Aurora A is present in a complex with D-TACC, which is a substrate for the kinase. We propose that one of the functions of Aurora A kinase is to direct centrosomal organization such that D-TACC complexed to the MSPS/XMAP215 microtubule-associated protein may be recruited, and thus modulate the behavior of astral microtubules.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1083/jcb.200108135DOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2173350/PubMed CentralArticle
ORCID:
AuthorORCID
Raff, Jordan W.0000-0002-4689-1297
Prigent, Claude0000-0001-8515-8699
Glover, David M.0000-0003-0956-0103
Additional Information:© 2002 The Rockefeller University Press. After the Initial Publication Period, RUP will grant to the public the non-exclusive right to copy, distribute, or display the Article under a Creative Commons Attribution-Noncommercial-Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/legalcode, or updates thereof. Received: 27 August 2001. Revised: 21 December 2001. Accepted: 24 December 2001. We would especially like to thank Adelaide Carpenter for her skilled tuition in carrying out electron microscopy on Drosophila larval brains, and Endre Mathe for helpful advice and stimulating discussions. We would like to thank the Cancer Research Campaign for a Program Grant to D.M. Glover. R. Giet was supported by Fellowships from the Marie Curie Program of the EEEC and the Human Frontiers Science Program. C. Prigent is supported by the Centre National de la Recherche Scientifique, L'association pour la Recherche sur le Cancer, and the Ligue Nationale Contre le Cancer. S. Deschamps is a fellow of the Ligue Nationale Contre le Cancer. J.W. Raff thanks the Wellcome Trust for a Senior Research Fellowship in Basic Biomedical Sciences, and M.J. Lee thanks the Medical Research Council for a research studentship.
Funders:
Funding AgencyGrant Number
Cancer Research CampaignUNSPECIFIED
Marie Curie FellowshipUNSPECIFIED
Human Frontier Science ProgramUNSPECIFIED
Centre National de la Recherche Scientifique (CNRS)UNSPECIFIED
L'association pour la Recherche sur le CancerUNSPECIFIED
Ligue Nationale Contre le CancerUNSPECIFIED
Wellcome TrustUNSPECIFIED
Medical Research Council (UK)UNSPECIFIED
Subject Keywords:Aurora A; D-TACC; mitosis; centrosomes; microtubule
Issue or Number:3
PubMed Central ID:PMC2173350
DOI:10.1083/jcb.200108135
Record Number:CaltechAUTHORS:20200807-170352216
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20200807-170352216
Official Citation:Régis Giet, Doris McLean, Simon Descamps, Michael J. Lee, Jordan W. Raff, Claude Prigent, David M. Glover; Drosophila Aurora A kinase is required to localize D-TACC to centrosomes and to regulate astral microtubules . J Cell Biol 4 February 2002; 156 (3): 437–451. doi: https://doi.org/10.1083/jcb.200108135
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:104854
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:13 Aug 2020 14:04
Last Modified:16 Nov 2021 18:37

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