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pavarotti encodes a kinesin-like protein required to organize the central spindle and contractile ring for cytokinesis

Adams, Richard R. and Tavares, Álvaro A. M. and Salzberg, Adi and Bellen, Hugo J. and Glover, David M. (1998) pavarotti encodes a kinesin-like protein required to organize the central spindle and contractile ring for cytokinesis. Genes and Development, 12 (10). pp. 1483-1494. ISSN 0890-9369. PMCID PMC316841. https://resolver.caltech.edu/CaltechAUTHORS:20200807-170353322

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Abstract

Mutations in the Drosophila gene pavarotti result in the formation of abnormally large cells in the embryonic nervous system. In mitotic cycle 16, cells of pav mutant embryos undergo normal anaphase but then develop an abnormal telophase spindle and fail to undertake cytokinesis. We show that the septin Peanut, actin, and the actin-associated protein Anillin, do not become correctly localized in pav mutants. pav encodes a kinesin-like protein, PAV–KLP, related to the mammalian MKLP-1. In cellularized embryos, the protein is localized to centrosomes early in mitosis, and to the midbody region of the spindle in late anaphase and telophase. We show that Polo kinase associates with PAV–KLP with which it shows an overlapping pattern of subcellular localization during the mitotic cycle and this distribution is disrupted in pavmutants. We suggest that PAV–KLP is required both to establish the structure of the telophase spindle to provide a framework for the assembly of the contractile ring, and to mobilize mitotic regulator proteins.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1101/gad.12.10.1483DOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC316841/PubMed CentralArticle
ORCID:
AuthorORCID
Glover, David M.0000-0003-0956-0103
Additional Information:© 1998 by Cold Spring Harbor Laboratory Press. The Authors acknowledge that six months after the full-issue publication date, the Article will be distributed under a Creative Commons CC-BY-NC License (Attribution-NonCommercial 4.0 International License, http://creativecommons.org/licenses/by-nc/4.0/). Received November 21, 1997; revised version accepted March 11, 1998. We are indebted to Christine Field and Gerald Rubin for kind donation of antibodies and to J. Hoheisel for provision of a gridded embryonic cDNA library. Yoshihiro Inoue helped with the P-element reversion analysis. We are grateful to Mar Carmena for critical reading of the manuscript. R.A. held a Medical Research Council (MRC) research studentship. Support for the project was provided by grants from the CRC, the MRC, the BBSRC, and the European Union. The publication costs of this article were defrayed in part by payment of page charges. This article must therefore be hereby marked “advertisement” in accordance with 18 USC section 1734 solely to indicate this fact.
Funders:
Funding AgencyGrant Number
Medical Research Council (UK)UNSPECIFIED
Cancer Research CampaignUNSPECIFIED
Biotechnology and Biological Sciences Research Council (BBSRC)UNSPECIFIED
European UnionUNSPECIFIED
Subject Keywords:Cytokinesis; kinesin-like protein; Polo kinase; Drosophila
Issue or Number:10
PubMed Central ID:PMC316841
Record Number:CaltechAUTHORS:20200807-170353322
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20200807-170353322
Official Citation:pavarotti encodes a kinesin-like protein required to organize the central spindle and contractile ring for cytokinesis Richard R. Adams, Alvaro A.M. Tavares, Adi Salzberg, Hugo J. Bellen, and David M. Glover Genes Dev. May 15, 1998 12: 1483-1494; doi:10.1101/gad.12.10.1483
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:104862
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:20 Aug 2020 17:27
Last Modified:20 Aug 2020 17:32

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