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The conserved Schizosaccharomyces pombe kinase plo1, required to form a bipolar spindle, the actin ring, and septum, can drive septum formation in G₁ and G₂ cells

Ohkura, Hiroyuki and Hagan, Iain M. and Glover, David M. (1995) The conserved Schizosaccharomyces pombe kinase plo1, required to form a bipolar spindle, the actin ring, and septum, can drive septum formation in G₁ and G₂ cells. Genes and Development, 9 (9). pp. 1059-1073. ISSN 0890-9369. doi:10.1101/gad.9.9.1059. https://resolver.caltech.edu/CaltechAUTHORS:20200807-170353435

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Abstract

We have identified a Schizosaccharomyces pombe gene with homology to the budding yeast gene CDC5, the Drosophila gene polo, and the mammalian family of genes encoding polo-like kinases. Disruption of this gene, plo1⁺, indicates that it is essential. Loss of plo1⁺ function leads to a mitotic arrest in which condensed chromosomes are associated with a monopolar spindle or to the failure of septation following the completion of nuclear division. In the latter case, cells show a failure both in the formation of an F-actin ring and in the deposition of septal material, suggesting that plo1⁺ function is required high in the regulatory cascade that controls septation. The overexpression of plo1⁺ in wild-type cells also results in the formation of monopolar spindles but also induces the formation of multiple septa without nuclear division. Septation can also be induced in the absence of mitotic commitment and concomitant spindle formation by the overexpression of plo1⁺ in cdc25-22 or cdc2-33 cells arrested in G₂; in G₁ cells arrested at Start by the cdc10-V50 mutation, or in cells lacking the cyclin B homolog cdc13 that undergo repeated S phases in the absence of mitosis.


Item Type:Article
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https://doi.org/10.1101/gad.9.9.1059DOIArticle
ORCID:
AuthorORCID
Glover, David M.0000-0003-0956-0103
Alternate Title:The conserved Schizosaccharomyces pombe kinase plo1, required to form a bipolar spindle, the actin ring, and septum, can drive septum formation in G1 and G2 cells
Additional Information:© 1995 by Cold Spring Harbor Laboratory Press. The Authors acknowledge that six months after the full-issue publication date, the Article will be distributed under a Creative Commons CC-BY-NC License (Attribution-NonCommercial 4.0 International License, http://creativecommons.org/licenses/by-nc/4.0/). Received January 20, 1995; revised version accepted March 16, 1995. We thank Paul Nurse and Jackie Hayles for S. pombe strains and genomic libraries, Kinsey Maundrell for the REP plasmid, Keith Gull for TAT1 antibody, Hans Lehrach for cosmid and P1 library filters, Alison Sparks and David Lane for help for flow cytometry, Emma Warbrick for consistently sound advice, and Fiona Cullen for help with DNA sequencing. This work is supported by the Cancer Research Campaign. The publication costs of this article were defrayed in part by payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 USC section 1734 solely to indicate this fact.
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Funding AgencyGrant Number
Cancer Research CampaignUNSPECIFIED
Subject Keywords:S. pombe; polo homolog; bipolar spindle; actin ring; septum; plo1 kinase
Issue or Number:9
DOI:10.1101/gad.9.9.1059
Record Number:CaltechAUTHORS:20200807-170353435
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20200807-170353435
Official Citation:The conserved Schizosaccharomyces pombe kinase plo1, required to form a bipolar spindle, the actin ring, and septum, can drive septum formation in G1 and G2 cells. H Ohkura, I M Hagan, and D M Glover Genes Dev. May 1, 1995 9: 1059-1073; doi:10.1101/gad.9.9.1059
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:104863
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:20 Aug 2020 17:43
Last Modified:16 Nov 2021 18:37

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